色谱2009，Vol.27Issue(6)：745-749,5.
桦褐孔菌子实体和发酵菌丝体中甾类化合物的定量测定
Quantitative determination of steroids in the fruiting bodies and submerged-cultured mycelia of Inonotus obliquus
摘要
Abstract
This study describes the method of quantitative determination of betulin,ergosterol,cholesterol,lanosterol,stigmasterol and sitosterol in the fruiting bodies and submerged-cultured mycelia of Inonotus obliquus. A high performance liquid chromatographic (HPLC) method was applied to separate these steroids. The procedure was carried out on a reversed-phase C_(18) column,using a stepwise gradient of water-methanol as mobile phase with the following profile: 0-10 min,10% water,90% methanol; 10-40 min,3% water,97% methanol. The flow rate was 1.4 mL/min and the detection wavelength was 202 nm. The analysis was completed within 40 min. The results showed that this method has good reproducibility and satisfactory recoveries for the determination of steroids. The relative standard deviations of the peak areas were less than 2.94% (n=5) for intraday assays. A good linear correlation was obtained in a range of 0.4-4.8 μg. The recoveries of betulin, ergosterol, cholesterol,lanosterol, stigmasterol,and sitosterol were 100.05% -100.72%,99.31% -101.04%,97.52% -101.63%,96.61% -100.08%,96.21% -100.76% and 100.04% -100.51%,respectively. This method can be applied to evaluate real samples,and it is rapid,accurate and suitable for the quantitative determination of steroids in the fruiting bodies and submerged-cultured mycelia of Inonotus obliquus.关键词
高效液相色谱/甾类化合物/子实体/菌丝体/桦褐孔菌Key words
high performance liquid chromatography (HPLC)/ steroids/ fruiting bodies/ mycelia/ Inonotus obliquus分类
化学化工引用本文复制引用
高远,许泓瑜,陆震鸣,许正宏..桦褐孔菌子实体和发酵菌丝体中甾类化合物的定量测定[J].色谱,2009,27(6):745-749,5.基金项目
This work was supported by National High-Tech Program of China (No. 2007AA021506),National Basic Research Program of China (No. 2007CB707804) and the Program for New Century Excellent Talents in the Universities of China (No. NCET-07-0380). （No. 2007AA021506）
