色谱2009，Vol.27Issue(6)：794-798,5.
柱前衍生反相高效液相色谱-荧光检测法测定大鼠血浆中的奈替米星
Determination of netilmicin in rat plasma by reversed-phase high performance liquid chromatography with fluorescence detection and pre-column derivatization
摘要
Abstract
A new, simple and sensitive method based on pre-column derivatization by reversed-phase high performance liquid chromatography (HPLC) is described for the separation and quantification of netilmicin in plasma, using 9-fluorenylmethyl chloroformate (FMOC-Cl) as the derivatization reagent. Its pharacokinetics is also presented. The derivatization modes and chromatographic conditions were optimized. The separation was performed on an Agilent ZOR-BAX Eclipse XDB-C_8 column (150 mm ×4. 6 mm, 5 μm) with a mixture of water-acetonitile (15:85, v/v) as mobile phase and the flow rate was 1.0 mL/min. The excitation wavelength was 265 nm and the emission wavelength was 315 nm. The linear range was 0. 045-8. 88 mg/L and the correlation coefficient (r) was 0. 999 3. The limit of detection (LOD) (S/N = 3) was about 0.01 mg/L, and the limit of quantification was 0.03 mg/L (3LOD) for netilmicin. The relative standard deviation was less than 3% for intra-day assay (n =5) and 3. 5% for inter-day assay (n = 5) and the relative recovery was in the range of 96. 62% - 100. 84% (n = 3). The plasma volume of 30 μL was sufficient for the determination of netilmicin. The method provides a reliable bioanalytical methodology to carry out netilmicin pharmacokinetics in rat plasma.关键词
柱前衍生化/氯甲酸芴甲酯(FMOC-Cl)/高效液相色谱/奈替米星/药代动力学/大鼠血浆Key words
pre-column derivatization/ 9-fluorenylmethyl chloroformate (FMOC-C1)/ high performance liquid chromatography (HPLC)/ netilmicin/ pharmacokinetics/ rat plasma分类
化学化工引用本文复制引用
常晓娟,彭敬东,刘绍璞,刘丽敏,代永矿..柱前衍生反相高效液相色谱-荧光检测法测定大鼠血浆中的奈替米星[J].色谱,2009,27(6):794-798,5.基金项目
国家自然科学基金项目(No. 20475045)和重庆市重点实验室资助项目(No. CSTC 2006CA8006). （No. 20475045）
