色谱2009，Vol.27Issue(6)：860-863,4.
胶束电动毛细管色谱法测定硫酸多粘菌素E药物中的多粘菌素E1和E2
Determination of polymyxin E1 and polymyxin E2 in polymyxin E sulfate using micellar electrokinetic capillary chromatography
摘要
Abstract
A method of micellar electrokinetic chromatography capillary ( MECC) has been established for separating polymyxins El and E2 in polymyxin E sulfate and determining the contents of El and E2. Several factors including the running voltage, the type of surfactant, concentrations of Bry-35 (polyoxyethyleneglycol dodecyl ether), NaCl solution and acetonitrile, pH of phosphate were investigated. Under the optimum conditions (10 kV running voltage, phos-phate buffer solution (0.01 mol/L, pH4. 1) containing 30 mmol/L Brrj-35, 5% (v/v) acetonitrile, 0. 167 mol/L NaCl), El and E2 were separated with the resolution of 1.94. The contents of El and E2 in polymyxin E sulfate were 67% and 32%, respectively. As an example, the relative standard deviations of the intra-assay and inter-assay of polymyxin El on the plate number and peak area were less than 5%. The method is simple, rapid, accurate, and reproducible.关键词
胶束电动毛细管色谱/多粘菌素E1/多粘菌素E2/硫酸多粘菌素EKey words
micellar electrokinetic capillary chromatography (MECC)/ polymyxin El/ poly-myxin E2/ polymyxin E sulfate分类
化学化工引用本文复制引用
闫永娜,王利娟,杨更亮,侯文欣,张巧霞..胶束电动毛细管色谱法测定硫酸多粘菌素E药物中的多粘菌素E1和E2[J].色谱,2009,27(6):860-863,4.基金项目
国家自然科学基金项目(Nos.20375010,20675084)、河北省科技攻关项目(Nos.06276479D,07276407D)和河北省教育厅项目(No. Z2007225). （Nos.20375010,20675084）
