生态学报2009，Vol.29Issue(11)：6142-6147,6.
化肥对稻田土壤细菌多样性及硝化、反硝化功能菌组成的影响
Effect of fertilization on bacterial community,genetic diversity of amoA and nosZ genes in paddy soil
摘要
Abstract
Polymerase Chain Reaction (PCR) and DNA sequencing techniques were applied to explore the effects of long-term fertilization on the community of bacteria and diversity of nitrifying and denitrifying bacteria in paddy soil. The analyses were based on the sequence libraries of 16S rDNA, amoA and nosZ genes. The Shannon Indices indicated that the diversity of soil bacteria and amoA gene of N treatment was lower than that of CK and NPK treatments, whereas the diversity of nosZ gene was opposite while the highest diversity occurred in N treatment. The LUBSHUFF statistical analyses demonstrated that both bacterial 16S rDNA and amoA gene libraries of CK, N and NPK treatments were significantly different from each other. As to the nosZ gene libraries, N and NPK treatments were remarkably different from CK, however, no significant difference was found between N and NPK treatments. It inferred that the application of chemical fertilizers influenced the community structure of bacteria and the functional genes of amoA and nosZ.关键词
水稻土/长期施肥/细菌多样性/amoA/nosZKey words
paddy soil/ fertilization/ bacterial diversity/ amoA/ nosZ分类
生物科学引用本文复制引用
陈哲,陈春兰,秦红灵,王霞,吴敏娜,魏文学..化肥对稻田土壤细菌多样性及硝化、反硝化功能菌组成的影响[J].生态学报,2009,29(11):6142-6147,6.基金项目
国家自然科学基金资助项目(40771115) （40771115）
中国科学院百人计划资助项目(KZCX2-YW-BR-01) （KZCX2-YW-BR-01）
国家支撑计划资助项目(2007BAQ010-47-7) （2007BAQ010-47-7）
