生物技术通报Issue(11):43-47,5.
蛋白质定向进化技术的研究进展
Research on Directed Evolution of Protein
摘要
Abstract
Directed evolution of protein is the effective strategy for the protein improvement. This review introduced the techniques for the mutation and recombination of DNA using error-prone PCR, DNA shuffling methods, and generation of mutational library and high throughput screening. Meanwhile, recent applications and development of directed evolution of protein were also reviewed in this paper.关键词
定向进化/易错PCR/DNA改组/高通量筛选Key words
Directed evolution/Error-prone PCR/DNA shuffling/High-throughput screening分类
生物科学引用本文复制引用
苏怡丹,伍宁丰,刘国安..蛋白质定向进化技术的研究进展[J].生物技术通报,2009,(11):43-47,5.基金项目
国家"863"计划项目(2007AA100605) (2007AA100605)