生物技术通报Issue(11)：79-82,4.
侧孢短芽孢杆菌X10启动子活性片段的克隆和序列分析
Cloning and Sequence Analysis of Fragments with Promoter Activity in Brevibacillus laterosporus X10
摘要
Abstract
High quality genomic DNA of Brevibacillus laterosporus X10 was extracted. The promoter library of X10 was constructed in Escherichia coli DH5α with pUC19-CFP as promoter vector by shotgun-cloning method. 14 positive clones designed as P1～P14 were obtained by screening, the fluorescent intensity of which was assayed to compare the promoting strength of the promoters. The results showed that the promoter of gjp in P6 was the strongest with fluorescent intensity of 3SS. 67 ,while the promoter of gfp in P14 was the weakest with fluorescent intensity of 211.67. The inserting fragments in the recombinant from P6 were sequenced and analyzed.关键词
侧孢短芽孢杆菌/启动子/克隆/序列分析Key words
Brevibacillus laterosporus/Promoter/Cloning/Sequence analysis分类
生物科学引用本文复制引用
李伟杰,姜瑞波,陈敏..侧孢短芽孢杆菌X10启动子活性片段的克隆和序列分析[J].生物技术通报,2009,(11):79-82,4.基金项目
国家科技资源平台项目(2005DKA21200) （2005DKA21200）
