生物技术通报Issue(11)：108-113,6.
甘菊cDNA-AFLP反应体系的优化
Optimization of cDNA-AFLP Technical System for Chrysanthemum lavandulifolium
摘要
Abstract
After systematical studies on the factors affecting cDNA-AFLP analysis system of Chrysanthemum lavandulifolium,an optimized system was established which showed a distinct electrophoretogram of cDNA-AFLP. The results indicated that using the improved Trizol method can isolate RNA quickly and efficiently from Chrysanthemum lavandulifolium; cDNA was digested and ligated by one step. The dosage of dscDNA was 300 ng. Digestion and ligation was performed in 8 hours; in 20 ul PCR reaction system of selective amplification, the primer concentration was 0. 4 mM; dNTP concentration was 0. 3 mM; Mg~(2+) concentration was 1. 25 mM; Taq DNA concentration was 0.9 U.关键词
甘菊/cDNA-AFLP/反应体系优化Key words
Chrysanthemum lavandulifolium/cDNA-AFLP/System optimization分类
农业科技引用本文复制引用
黄河,王顺利,曹华雯,戴思兰..甘菊cDNA-AFLP反应体系的优化[J].生物技术通报,2009,(11):108-113,6.基金项目
国家高技术研究发展计划("863"计划)重点项目(2007AA021403) （"863"计划）
