生物技术通报Issue(11)：114-116,3.
野生山梨基因组DNA提取方法和部位比较
Comparison of DNA Extraction Methods in Different Parts of Wild Pyrus
摘要
Abstract
Genomic DNA were extracted by CTAB, improved CTAB + 3M NaAc +5%PVP and DNA extraction Kit from young leaves,phloem of one-year-old shoot and maturing leaves of wild Pyrus ussuriensis Maxim. The OD value of DNA were measured, and DNA agarose gel electrophoresis and PCR amplification of SSR were tested the results showed phloem and young leaves are the best ideal material for DNA extraction,improved CTAB +3M NaAc +5% PVP is a better method for DNA extraction,and DNA extraction Kit is the most simple,quickly and effective method.关键词
野生山梨/基因组DNA/SSRKey words
Wild Pyrus/Genomic DNA/SSR分类
农业科技引用本文复制引用
田路明,高源,曹玉芬..野生山梨基因组DNA提取方法和部位比较[J].生物技术通报,2009,(11):114-116,3.基金项目
中国农业科学院公益性科研院所基本科研业务费专项(082060302-08) （082060302-08）
