生物技术通报Issue(11)：125-129,5.
单克隆抗体与多克隆抗体配对ELISA方法比较
Study of the Optimum Pairing of Polyclonal Antibodies and Monoclonal Antibodies with Sandwich ELISA
张小兵 1邸禄芹 2吴萌 1闫静辉1
作者信息
- 1. 河北省生物研究所,石家庄050081
- 2. 河北医科大学第三医院,石家庄050051
- 折叠
摘要
Abstract
Using human chorionic gonadotrophin( HCG) as the antigen,polyclonal antibodies and monoclonal antibodies(McAbs) specific for HCG were generated, characterized and highly purified. Then these antibodies were labeled with horseradish peroxidase (HRP). By a double antibody sandwich enzyme-linked immunosorhent assay, several problems of the optimum pairing of polyclonal antibodies and monoclonal antibodies were discussed. The results showed that with monoclonal antibody as trapping antibody and polyclonal antibody labeling with enzyme as testing antibody which should be diluted with animal sera as diluent,the sandwich ELISA had a highly specificity and sensitivity to detect antigen.关键词
单克隆抗体/多克隆抗体/抗体配对/ELISA法Key words
Monoclonal antibody/Polyclonal antibody/Optimum pairing of antibodies/Sandwich ELISA分类
生物科学引用本文复制引用
张小兵,邸禄芹,吴萌,闫静辉..单克隆抗体与多克隆抗体配对ELISA方法比较[J].生物技术通报,2009,(11):125-129,5.
