山西大学学报（自然科学版）2009，Vol.32Issue(3)：455-462,8.
小麦中源于中间偃麦草抗白粉病基因PmCH5026的SSR定位
Mapping of a Thinopyrum intermedium-derived Powdery Mildew Resistance Gene PmCH5026 in Wheat with SSR Markers
摘要
Abstract
Powdery mildew,caused by Blumeria graminis f. sp. tritici,is one of the most important diseases of wheat (Triticum aestivum L. ) worldwide and causes severe yield losses. Breeding for resistance is the most economical and effective method for controlling the disease. The wheat breeding line CH5026 derived from the partial wheat-Thinopyrum intermedium amphiploid TAI7045 is resistant to powdery mildew. To determine inheritance of powdery mildew resistance and map the resistance gene(s) in CH5026,F_2 ,F_3, BC_1F_2 progenies derived from the crosses of CH5026 with line CH5065 and susceptible cv. Jintai 170 were inoculated with Bgt isolate E09 in the greenhouse. Genetic analysis indicated that a single dominant gene, temporarily designated PmCH5026, was responsible for the resistance to Bgt in line CH5026. A total of 378 SSR markers were used to test the parents and resistant and susceptible bulks from the F_2 segregating population of CH5026 × CH5065. From the bulked segregant analysis,the polymorphic SSR markers were selected for genotyping the F_2 population. Three SSR markers Xcfd233 , Xbarc11 and Xgwm539 were identified to be linked to the resistance gene and their most likely order was Xcfd233,7. 2 cM, PmCH5026, 4.9 cM, Xbarc11 , 5. 5 cM, Xgwm539. Using the Chinese Spring nullisomic-tetrasomic and ditelosomic lines,the SSR markers mapped the resistance gene on chromosome arm 2DL.关键词
小麦/中间偃麦草/白粉病/基因定位/SSRKey words
wheat/Thinopyrum intermedium /powdery mildew/gene mapping/SSR分类
农业科技引用本文复制引用
贺润丽,畅志坚,刘建霞,詹海仙,张晓军,董春林..小麦中源于中间偃麦草抗白粉病基因PmCH5026的SSR定位[J].山西大学学报（自然科学版）,2009,32(3):455-462,8.基金项目
国家自然科学基金(30671299,39870398) （30671299,39870398）
山西省科技攻关计划项目 （）
