陕西师范大学学报（自然科学版）2009，Vol.37Issue(6)：68-70,3.
适于AFLP分析的华中五味子DNA提取方法改进
A modified method of DNA extraction from Schisandra sphenanthera Rehd. et Wils for AFLP analysis
摘要
Abstract
To obtain high quality genomic DNA isolated from dry leaves of Schisandra sphenantheraRehd, et Wils for AFLP analysis. A modified CTAB method was developed, which includes dissolving plant tissue in CTAB-free buffer before isolating the nucleolus and removing polysaccharide with high salt. The results showed that the value of OD_(260)/OD_(280) was from 1.80 to 2.00, main bands were clear, and degradation was nearly free. Genome DNA was suitable for digestion by restriction endonucleases (EcoR Ⅰ and Mse Ⅰ). AFLP analysis indicated the patterns were clear and polymorphic, indicating that this method could be used for extracting ideal DNA from dry leaves of Schisandra sphenanthera Rehd. et Wils and suitability for AFLP analysis.关键词
华中五味子/DNA提取/CTABKey words
Schisandra sphenanthera Rehd. et Wils/ DNA isolation/ CTAB分类
生物科学引用本文复制引用
刘锦,罗成,顾蔚,王喆之..适于AFLP分析的华中五味子DNA提取方法改进[J].陕西师范大学学报（自然科学版）,2009,37(6):68-70,3.基金项目
国家"十一五"科技支撑项目(2006BAI06A13-06) （2006BAI06A13-06）
