天然产物研究与开发2009，Vol.21Issue(5)：771-775,5.
青钱柳多糖对小鼠骨髓来源树突状细胞表面分子表达的影响
Effect of Cyclocarya paliurus (Batal.) Polysaccharides on the Surface Molecule Expression Level of Dendritic Cells
摘要
Abstract
To observe the effect of Cyclocarya paliurus(Batal. )polysaccharides(CPC)on differentiation and development of murine bone marrow derived dendritic cells (DCs) in vitro. Monocytes generated from Balb/c mouse were cultured in completed RPMI1640 medium containing 10%FBS,rmGM-CSF and rm IL-4.The morphology development was observed by invented microscope and transmission electron microscope. DCs on the 6th day were analyzed by flow cytometry ( FCM) to determine the expression levels of CD80,CD86 and MHC II. CPC was added to cells on day 6 of culture for 24 h, then the surface molecules expression level of MHC II on DCs were analysis by FCM. The results showed that DCs induced by rmGM-CSF and rm IL-4 possessed typical morphology and phenotype on the 6 day,which can be used in the following experiments. Compared to untreated DCs, DCs treated with CPC increased the expression leves of MHC II, which indicated that CPC could promote the phenotypic maturation of DCs.关键词
青钱柳多糖/树突状细胞/表型/成熟/流式细胞术Key words
Cyclocarya paliurus (Batal. ) polysaccharides/ dendritic cells/ phenotypic/ maturation/ flow cytometry分类
医药卫生引用本文复制引用
黄丹菲,聂少平,谢建华,韩澄,谢明勇..青钱柳多糖对小鼠骨髓来源树突状细胞表面分子表达的影响[J].天然产物研究与开发,2009,21(5):771-775,5.基金项目
国家自然科学基金(No.30660226) （No.30660226）
教育部长江学者和创新团队发展计划(No.IRT0540) （No.IRT0540）
食品科学与技术国家重点实验室目标导向项目(SKLF-MB-200806) （SKLF-MB-200806）
