天然产物研究与开发2009，Vol.21Issue(5)：792-795,4.
菜油基长链辣椒素酯小型化合物库的制备与初步生物活性
Synthesis of a Small Library of Long-chain Capsinoids with Acyl Moiety of Colza Oil and Their Preliminary Bioactivity
摘要
Abstract
In this paper,a small library of long-chain capsinoids(LCs)with acyl of colza oil was synthesized directly by transesterification of vanillyl alcohol with colza oil using lipase as catalyst in organic media. The mixture of 0.5 g immobilized lipase Novo 435 and 100 mL acetone solution containing 5 mmol vanillyl alcohol and 2. 5 mmol colza oil was shaken for 24 h in a temperature-controlled incubator shaker at 200 r/min and 30 ℃. The product was purified by silica gel chromatography with 34.1% yield and a small natural long-chain capsinoids library, which was composed of vanillyl linolenate(1), vanillyl linoleate(2) ,vanillyl palmitate(3) ,vanillyl oleate(4) ,vanillyl stearate(5) ,vanillyl eicosenoate (6) and vanillyl docosenoate(7) mainly,was established and displays a low activity as a PPARγ agonist with activity times of 1.5 at 50 μg/mL. Compounds 1,2,3,4,7 were separated from the LCs by preparative reversed-phase high performance liquid chromatography and characterized by ~1H NMR and compound 7 was first reported.关键词
辣椒素酯/脂肪酶/酶催化合成/有机介质/菜子油Key words
capsinoid/lipase/enzymatic synthesis/organic media/colza oil分类
农业科技引用本文复制引用
董新荣,刘仲华,李雨虹,谢达平,何理..菜油基长链辣椒素酯小型化合物库的制备与初步生物活性[J].天然产物研究与开发,2009,21(5):792-795,4.基金项目
国家科技部十五科技重点项目(No.2004BA542C) （No.2004BA542C）
湖南省自然科学基金(07JJ6026) （07JJ6026）
