天然产物研究与开发2009，Vol.21Issue(5)：806-812,7.
液相色谱/离子阱质谱法研究何首乌中糖苷类化合物
HPLC/IT-MS Analysis of Glycosides in Radix Polygoni multiflori
摘要
Abstract
HPLC/MS was developed for analysis and identification of glycosides in radix Polygoni multiflori, and the process of ionization of molecular ion of physcion glycoside and quasi-molecular ion of emodin glycoside were studied. Samples were analyzed by a reversed-phase C_(18) column using a binary eluent under gradient conditions,and twelve components were isolated. A collision induced dissociation (CID) experiment was carried out in order to identify the structure of glycosides. The structures of nine glycosides were inferred by MS and information from reports. Three of them: cis-2,3, 5,4'-tetrahydroxystilbene-2-O -β-D-glucopyranoside, torachrysone-8-O-(6'-O-acetyl) -β-D-glucopyranoside, physcion-8-O-(6'-O-acetyl)-B-D-glucopyranoside have not been reported until now.关键词
液相色谱/质谱联用(HPLE/MS)/离子阱(IT)/大气压化学电离源(APCI)/何首乌/糖苷Key words
high performance liquid chromatography/mass spectrometry( HPLC/MS)/ ion trap( IT)/ atmospheric-pres-sure chemical ionization(APCI )/radix Polygoni mukiflori/glycosides分类
医药卫生引用本文复制引用
孙晋苓,黄晓兰,吴惠勤,黄芳..液相色谱/离子阱质谱法研究何首乌中糖苷类化合物[J].天然产物研究与开发,2009,21(5):806-812,7.基金项目
广东省科技计划项目(2006B35605009) （2006B35605009）
