天然产物研究与开发2009，Vol.21Issue(5)：817-821,848,6.
HPLC-DAD和LC-MS/MS法对各种芦荟样品中蒽醌类成分的分析研究
Analysis of Anthraquinones in Different Samples of Aloe with HPLC-DAD and LC-MS/MS
摘要
Abstract
A HPLC-DAD and LC-MS/MS method was established not only for the assay of anthraquinones but also for the determination of aloin and aloe-emodin in different samples of aloe. The analysis was performed on Nucleodur-silica C_(18)(250 mm *4.6 mm,5 μm)column with a methanol-water (containing 0.4% acetic acid) gradient at ambient temperament. The flow rate was 1.0 mL/min, the wavelength scanned from 190-370 nm and the detection was at 254 nm and 356 nm. The ionic source of LC-MS was ESI. Samples were analyzed in two modes: MS and MS/MS full can modes. Aloin A,B and aloe-emodin were the main anthraquinones in different samples of aloe while rhein,emodin,chrysophanol and physcion were not found; the aloe powder contained the highest amount of aloin. The method could be used to control the quality of aloe product.关键词
芦荟/芦荟苷/芦荟大黄素/HPLC-DAD/LC-MS/MSKey words
aloe/ aloin/ aloe-emodin/ HPLC-DAD/ LC-MS/MS分类
医药卫生引用本文复制引用
陈欣霞,张宇航,林颖,谢志勇,万金志..HPLC-DAD和LC-MS/MS法对各种芦荟样品中蒽醌类成分的分析研究[J].天然产物研究与开发,2009,21(5):817-821,848,6.基金项目
2005年度广东省中医药管理局计划项目(1050163) （1050163）
2005年度珠海市科技计划项目(PC20051072) （PC20051072）
