天然产物研究与开发2009，Vol.21Issue(5)：849-853,5.
双水相系统纯化山楂叶中黄酮类物质的研究
Research of Aqueous Two-phase Extraction System Purification Flavonoids of Hawthorn Leaves
赵立辉 1武文洁 1杨昱涵1
作者信息
- 1. 天津科技大学应用化学专业,天津,300457
- 折叠
摘要
Abstract
Aqueous two-phase extraction system (ATPE) which formed with water, alcohol and ammonium sulfate,is used to purify flavonoids substance and remove hydrophilic substance. This experiment identified the different influence factors of purification effect which included different salt,different volume rate of water and alcohol and addition of salt. Ammonium sulfate had the best parvafacies effect, and 2. 5 g ammonium sulfate just can satisfy the parvafacies. (1) In the ATPE of 5 mL alcohol + 5 mL distilled water + 5 g ammonium sulfate +0.15 g crude extraction( the content of crude extraction is 25% ) .recovery percent of flavonoids substance is 89.2% ,and the percent of flavonoids is 47. 6% in the purification product. (2)In the ATPE of 4 mL alcohol + 5 mL distilled water + 5 g ammonium sulfate +0.15 g crude extraction (the content of crude extraction is 25% ) , recovery percent of flavonoids substance is 81.9% , and the percent of flavonoids substance is 50.9% in the purification product. Literatures which purify the flavonoid substance of hawthorn leaves with ATPE including water, alcohol and ammonium sulfate, are seldom reported. This experiment using ATPE method can enhance the percent of flavonoid from 25% to 47.6% ,and the recovery percent is 89%.关键词
双水相萃取系统/黄酮/硫酸铵/纯化Key words
aqueous two-phase extraction system (ATPE)/ purification/ flavonoid/ ammonium sulfate分类
农业科技引用本文复制引用
赵立辉,武文洁,杨昱涵..双水相系统纯化山楂叶中黄酮类物质的研究[J].天然产物研究与开发,2009,21(5):849-853,5.
