生态环境学报2009，Vol.18Issue(5)：1669-1673,5.
铅暴露与排放对中华鲟幼鱼血液中ALT、AST活力的影响
Effects of lead on activity of ALT and AST in serum of juvenile Chinese sturgeon, Acipenser sinensis
摘要
Abstract
The disruption of Alanine amiotransferase (ALT) and Aspartate aminotransferase (AST) activity in serum of juvenile Chinese sturgeon (Acipenser sinensis) caused by chronic waterborne Pb was demonstrated in the present study. Fish (from 96 hours developed zygotes to juvenile) were exposed to 0 mg·L~(-1) (control), 0.2 mg·L~(-1), 0.8 mg·L~(-1) and 1.6 mg·L~(-1) Pb~(2+) for 16 weeks and then transferred to Pb-free water for 6 weeks. Results showed that, after long term lead exposure, ALT and AST activity presented a up-ward trend with the increasing Pb concentrations. Significant increase of ALT activity was only found in 1.6 mg·L~(-1) group (P<0.01) while significant increase of AST activity was found both in 0.8 mg·L~(-1) group (P<0.05) and1.6 mg·L~(-1) group (P<0.01). After de-puration in clean water for 6 weeks, ALT activity of juveniles exposed to all lead groups decreased to the level of control group(P>0.05), however, the activity of AST was still high, almost twice higher than control when exposed in the concentration of 1.6 mg·L~(-1) Pb~(2+). AST: ALT ratio of control group was from 2.24 to 2.32. After Pb exposure, ALT: AST ratio presented a upward trend with in-creasing Pb concentrations. After Pb depuration, AST: ALT ratio decreased, however, the ratio in 1.6 mg·L~(-1) group was still higher than control group(P<0.05). It was considered that after exposure in 0.2 mg·L~(-1) or 0.8 mg·L~(-1) Pb~(2+) concentration, liver of juvenile Chinese sturgeon maybe impaired in different degrees and slight damage could be repaired after depurated in clean water. AST: ALT ratio was also important for evaluating the impairment of fish tissue.关键词
铅/中华鲟/暴露/排放/丙氨酸氨基转移酶(ALT)/天冬氨酸氨基转移酶(AST)/恢复Key words
Lead/ Chinese sturgeon/ exposure/ depuration/ ALT/ AST/ recovery分类
资源环境引用本文复制引用
侯俊利,庄平,冯琳,章龙珍,张涛,冯广朋,刘鉴毅,徐滨..铅暴露与排放对中华鲟幼鱼血液中ALT、AST活力的影响[J].生态环境学报,2009,18(5):1669-1673,5.基金项目
国家自然科学基金重大项目课题(30490234) （30490234）
国家高技术研究发展计划项目(2008AA10Z227) （2008AA10Z227）
中央级公益性科研院所基本科研业务费专项资金项目(2007M023,2007M01,2008M01) （2007M023,2007M01,2008M01）
上海市长江口中华鲟自然保护区项目 （）
