中国组织工程研究与临床康复2009，Vol.13Issue(37)：7249-7252,4.DOI:10.3969/j.issn.1673-8225.2009.37.009
退变椎间盘的细胞培养
Cell culture of human degenerative intervertebral disc
马可 1胡祖鹏 1齐宗华 2胡有谷2
作者信息
- 1. 复旦大学附属华山医院急诊科,上海市,200040
- 2. 青岛大学医学院骨科研究室,山东省,青岛市,266003
- 折叠
摘要
Abstract
BACKGROUND: There are no reports about adult degenerative disc cell culture model currently. This establishment of disc cell culture is the cellular basis of intervertebral disc tissue engineering. OBJECTIVE: To establish cell culture models from human degenerative intervertebral disc, and to settle the cytology base of disc tissue engineering. DESIGN AND SETTING: The controlled observational experiment with regard to cytology was performed in Shandong Institute of Orthopaedics and Traumatology. MATERIALS: Five degenerative intervertebral disc patients were recruited from Department of Orthopaedics, Affiliated Hospital of Qingdao University Medical College, including one case in L2-3, three cases in L4-5 and one case in L5-S1. METHODS: Five samples were taken from the operational disectomy for lumbar disc herniation, and these samples were cultured using enzymatic digestion cell culture and tissue cell culture method differently. All the samples were cultured with HAMF12 medium with the addition of 10% and 20% fetal bovine serum. The morphology of the cultured cell was observed with Giernsa staining and transmission electron microscopy respectively. MAIN OUTCOME MEASURES: ①Cellular growth; ②Morphology of the cultured cells;③Ultrastructure Of the cultured cells. RESULTS: By means of enzymatic digestion cell culture and tissue cell culture method, numerous degenerative lumbar disc cells were obtained and successfully subcultured. The cell secretions around the cultured cells may influence the growth of these cells. Destroying the secretions, the cultured cells could be highly proliferated. The degenerative intervertebral disc cells were proliferated vigorously in HAMF12 medium with 20% fetal bovine serum compared with that with 10% fetal bovine serum. The notochordal cell was observed in all the specimens. CONCLUSION: The adult degenerative disc can be used to obtain the required cell for tissue engineering.关键词
退变椎间盘/椎间盘细胞/细胞培养/脊索细胞分类
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马可,胡祖鹏,齐宗华,胡有谷..退变椎间盘的细胞培养[J].中国组织工程研究与临床康复,2009,13(37):7249-7252,4.
