中国组织工程研究与临床康复2009，Vol.13Issue(40)：7979-7984,6.DOI:10.3969/j.issn.1673-8225.2009.40.035
骨髓间充质干细胞移植治疗大鼠心肌梗死及随后的心肺复苏
Cardiopulmonary resuscitation in myocardial infarction rats treated with bone marrow mesenchymal stem cell transplantation
摘要
Abstract
BACKGROUND:The majority of published article on cardiopulmonary resuscitation (CPR) used healthy animals. In fact, patients commonly have severe heart diseases before CPR, leading to ventricular fibrillation. OBJECTIVE: To investigate outcome of myocardial function and cardiopulmonary resuscitation in myocardial infarction rats treated with bone marrow mesenchymal stem cells (MSCs) transplantation.DESIGN, TIME AND SETTING: A randomized, controlled animal experiment was performed at the University of Southern California and Second Hospital of Sun Yat-sen University from April to August 2007.MATERIALS: A total of 18 adult male SD rats were randomly divided into model control and cell transplantation groups with 9 animals in each group. In addition, 1 SD rat aged 1 month was used to prepare bone marrow MSCs.METHODS: Myocardial ischemia was induced by ligation of the left anterior descending artery (LAD). Animals respectively received 5×106 MSCs (0.1 mL) marked with PKH26 in phosphate buffer solution (PBS) or PBS alone 4 weeks after LAD ligation. Ventricular fibrillation and CPR were performed 4 weeks after MSCs or PBS injection.MAIN OUTCOME MEASURES: Heart function was evaluated by ultrasound cardiography 2, 4 weeks after transplantation; hemodynamics was measured before and 4 hours following CPR. Myocardial tissues were harvested 72 hours after CPR for pathological exanimation.RESULTS: Compared with model control group, ejection fraction of transplantation group was significantly increased 2 and 4 weeks after transplantation (P<0.01), and cardiac index, dp/dt40, and -dp/dt were significantly improved before and within 4 hours after CPR (P<0.01, P<0.05). Moreover, the rats survived longer in transplantation group (72 hours) after CPR compared with control group (P<0.05). Pathological section results showed a large number of PKH26-1abeled MSCs in the rnyocardium.CONCLUSION: Myocardial function, hemodynamics and survival time after CPR were significantly improved in animals treated with MSCs transplantation.关键词
骨髓间充质干细胞/心肌梗死/心功能/心肺复苏分类
基础医学引用本文复制引用
王彤,伍权华,万智,黄辉,翁胤仑..骨髓间充质干细胞移植治疗大鼠心肌梗死及随后的心肺复苏[J].中国组织工程研究与临床康复,2009,13(40):7979-7984,6.基金项目
广东省自然科学基金项目(8151008901000119) （8151008901000119）
2009年中山大学青年人才基金(B002009006) the Natural Science Foundation of Guangdong Province No.8151008901000119 the Excellent Youth Talent Foundation of Sun Yat-sen University in 2009,No.B002009006 （B002009006）
