中国组织工程研究与临床康复2009，Vol.13Issue(42)：8281-8285,5.DOI:10.3969/j.issn.1673-8225.2009.42.018
热压-盐析法制备骨组织工程支架的工艺及性能表征米
Technology and characterization of bone tissue engineering scaffold using hot-pressing and particulates leaching process
摘要
Abstract
BACKGROUND: Hot-pressing and particulates-leaching is a convenient method to construct bone tissue engineering scaffold and the properties of the constructed scaffold are well. Both process and materials have effect on the properties of the constructed scaffold. OBJECTIVE: To investigate the influence of content of hydroxyapatite (HA), time, temperature and pressure of the hot-pressing on the properties of the scaffold.DESIGH, TIME AND SETTING: A bone tissue engineering scaffold construction experiment was performed at Institute of Nano and Bio-Polymeric Materials, School of Material Science and Engineering, Tongji University from September 2008 to May 2009. MATERIALS: Polylactic acid (PLA), polycaprolactam (PCL) and HA were synthesised in our laboratory. METHODS: The PLA/PCL/nHA bone tissue engineering scaffold was fabricated via hot-pressing and particulates leaching. Briefly, the PLA and PCL with weight ratio of 7:3 were dissolved into chloroform to form polymer solution. nHA was dispersed into the polymer solution after ultrasonic vibration. Then, sodium chloride was added into the solution to obtain a composite. After the chloroform was removed by evaporation and vacuum-dried, the composite were charged into a mold. MAIN OUTCOME MEASURES: ①The phase, shape and size of HA was investigated by XRD and transmission electron microscope.②Morphology of scaffold was investigated by scanning electron microscope. ③Hydrophilicity was investigated by surface contact angle. ④Influence of hot-pressing time, temperature and pressure on the porosity and compressive strength of the scaffold. ⑤Influence of different content of HA on the compressive strength and surface contact angle of scaffold. RESULTS: The result showed that the pores of PLA/PCL/nHA scaffold were in diameter of 300-340 urn and interconnected. The surface of the scaffold was also interconnected pores, but not the structure like "skin". The porosity and compressive strength met the requirements of the application of the scaffold. The compressive strength of the scaffold contained HA increased while the water absorption decreased. The properties of the scaffold were much better when the content of the HA was 4%. Moreover, the time, temperature and pressure also affected the properties of the scaffold. The result showed that the properties of scaffold was much better when it was fabricated under holding the pressure of 7 MPa at 65℃ for 3 minutes. CONCLUSION: The bone tissue engineering scaffold constructed by hot-pressing and particulates-leaching can meet the needs of the application. The hot-pressing temperature, time and pressure do have effect on the properties of the scaffold. HA can enhance the compressive strength of the scaffold; moreover, it decreases the hydrophilicity of the scaffold.关键词
多孔支架/骨组织工程/热压-盐析法/纳米羟基磷灰石/支架性能分类
医药卫生引用本文复制引用
任天斌,宋莹,冯玥,袁伟忠..热压-盐析法制备骨组织工程支架的工艺及性能表征米[J].中国组织工程研究与临床康复,2009,13(42):8281-8285,5.基金项目
国家自然科学基金项目(30800230/C100202) （30800230/C100202）
上海市科委纳米专项(0852nm03600) （0852nm03600）
