畜牧兽医学报2009，Vol.40Issue(11)：1621-1629,9.
荷斯坦母牛TLR6基因多态性及其与体细胞评分关系的研究
Polymorphism of TLR6 Gene and Its Relationship with Somatic Cell Score in Holstein Cows
摘要
Abstract
The objective of the present study was to elucidate the relationship between Toll-like re-ceptor 6(TLR6)gene and mastiffs resistance of Holstein cows. Polymorphism of all exons of TLR6 gene was detected in 208 Holstein cows by PCR-SSCP and PCR-RFLP. The relationship between polymorphisms of TLR6 gene and somatic cell score (SCS) in Holstein cows was ana-lyzed by least squares linear model. Six polymorphic sites T853A, G855A, G1793A, C1859A, G1934A and A1980G were found; and T853A mutation resulted in an amino acid change of valine →glutamic, G855A mutation caused an amino acid change of aspartic acid→asparagine, A1980G mutation caused an amino acid change of isoleucine→valine. For T853A and G855A polymorphic sites, three genotypes (AA, AB and BB) were found in 208 Holstein cows by SSCP, and the fre-quency of AA, AB and BB genotypes was 0.308, 0.490 and 0.202, respectively. Holstein cows were in Hardy-Weinberg equilibrium at the sites. Least squares mean of SCS for BB was signifi-cantly lower than that for AB (P<0.01) or AA (P<0.01), least squares mean of SCS for AB was significantly lower than that for AA (P<0.01). For G1793A polymorphic site, two geno-types (CC and CD) were found by digesting the PCR products with restriction endonuclease BsiY Ⅰ, Chi-square test showed that Holstein cows were not in Hardy-Weinberg equilibrium. Least squares mean of SCS for CC was not significantly lower than that for CD (P>0.05). For C1859A polymorphic site, two genotypes (EE and EF) were found by digesting the PCR products with re-striction endonuclease BstX Ⅰ, and the frequency of EE and EF genotypes was 0.178 and 0.822, respectively. Holstein cows were not in Hardy-Weinberg equilibrium at the site. Least squares mean of SCS for EE was not significantly lower than that for EF (P>0.05). For G1934A poly-morphic site, two genotypes (GG and GH) were found by digesting the PCR products with re-striction endonuclease BsiY Ⅰ, and the frequency of GG and GH genotypes was 0.356 and 0.644, respectively. Holstein cows were not in Hardy-Weinberg equilibrium at the site. Least squares mean of SCS for GG was not significantly lower than that for GH (P>0.05). For A1980G poly-morphic site, three genotypes (II, IJ and JJ) were found by digesting the PCR products with re-striction endonuclease Bcl Ⅰ, and the frequency of II, IJ and JJ genotypes was 0.260, 0.567 and 0.173, respectively. Holstein cows were in Hardy-Weinberg equilibrium. Least squares mean of SCS for JJ was significantly lower than that for IJ (P<0.01) or II (P<0.01), least squares mean of SCS for IJ was significantly lower than that for II (P<0.01). BB and JJ were the most favorable genotypes, AA and II were the most unfavorable genotypes for mastitis resistance. The results preliminarily indicated that both B and J alleles of TLR6 gene are two potential DNA markers for improving mastitis resistance in dairy cattle.关键词
荷斯坦母牛/TLR6基因/多态性/乳房炎/体细胞评分Key words
Holstein cows/ TLR6 gene/ polymorphism/ mastitis/ somatic cell score分类
农业科技引用本文复制引用
储明星,李春苗,石万海,安永福,陈宏权,狄冉,方丽..荷斯坦母牛TLR6基因多态性及其与体细胞评分关系的研究[J].畜牧兽医学报,2009,40(11):1621-1629,9.基金项目
国家"十一五"科技支撵计划课题(2006BAD04A10) （2006BAD04A10）
北京市自然科学基金项目(6022015) （6022015）
