畜牧兽医学报2009，Vol.40Issue(11)：1638-1644,7.
高锌对早期断奶仔猪肠黏膜屏障和肠上皮细胞紧密连接蛋白表达的影响
Effect of High Level of Zinc Oxide on Tight Junction Protein Expression in Intestinal Epithelial Cells and Intestinal Mucosal Barrier in Early Weaning Piglets
摘要
Abstract
To study the effect of high level of zinc oxide on intestinal mucosal barrier and its mech-anisms in early weaning piglets, 54 piglets, weaned at 21 d of age, were allocated into three treatments on the basis of similar BW, ancestry, and gender. Each treatment had three replica-tions of six pigs (half males and half females). The experimental treatments diets were basal diet and basal diet +3 000 mg·kg~(-1) Zn (ZnO), another treatment piglets were receiving sow milk with mother (control). Plasma D-lactate and endotoxin level, DAO activity, bacterial transloca-tion in mesenteric lymph nodes and colon E. coli were measured to evaluate the intestinal permea-bility at 28, 35 d of age, and the level of Occludin and ZO-1 expression were also detected. The results showed that plasma D-lactate and endotoxin level and DAO activity were significantly in-creased in piglets of basal diet compared to the control group (P<0.05) at 28 d. The three pa-rameters remained high at 35 d. There were no significant differences between the high zinc and the control (P>0.05). At 28 and 35 d, bacterial translocation in mesenteric lymph nodes of pig-lets with high zinc was lower than that of piglets with basal diet(P>0.05). Colon E. coli of pig-lets with basal diet was increased compared to the control group (P<0.05) at 28 d, but there were no significant difference between the high zinc and the basal diet (P>0.05). The parameters did not differ among three groups at 35 d (P>0.05). Compared with the control, Occludin and ZO-1 mRNA and ZO-1 expression was significantly decreased in the basal diet group (P<0.05) and the high zinc (P>0.05), but the parameters of piglets with high zinc was greater than that of basal diet (P<0.05). It is suggested that early weaned piglets was in stresses, increasing in-testinal permeability and decreasing the expression of tight junction protein Occludin and ZO-1, and these damage may be relaxed by diet supplementation with high level of zinc oxide, through inhibiting bacterial adhesion in the intestinal mucosa and preventing the increase in epithelial cells permeability.关键词
高锌/早期断奶/仔猪/肠黏膜屏障/紧密连接蛋白Key words
high level of zinc oxide/ early weaning/ piglets/ intestinal mucosal barrier/ tight junc-tion protein分类
农业科技引用本文复制引用
胡彩虹,钱仲仓,刘海萍,徐勇..高锌对早期断奶仔猪肠黏膜屏障和肠上皮细胞紧密连接蛋白表达的影响[J].畜牧兽医学报,2009,40(11):1638-1644,7.基金项目
浙江省自然科学基金资助项目(Y306028) （Y306028）
浙江省重大科技专项农业项目(2008C12087) （2008C12087）
