畜牧兽医学报2009，Vol.40Issue(11)：1651-1657,7.
牛IL-2重组质粒对口蹄疫病毒G-H环多肽免疫小鼠的佐剂效应
Effects of Recombinant Bovine IL-2 on Immune Response of FMDV G-H Loop Peptide in Mice
摘要
Abstract
To study the immune response induced by G-H loop peptide of Asial foot-and-mouth disease virus (FMDV) with BOIL-2 recombinant plasmid as adjuvant, the immune effects in im-munized mice were investigated. Recombinant plasmids pcDNA3.1-pBoIL-2 was constructed and the condition of the expression was identified in vitro. G-H peptide was expressed in Rosetta (DE3)pLysS and were successfully purified through GST affinity purification. Five groups of Kunming mice each with 8 were inoculated with different combinations including PBS, pcD-NA3.1-pBoIL-2 and/or G-H peptide, subsequently, immune effects have been evaluated through indirect ELISA assay, the neutralization test, lymphocytes proliferation assay and Quantitive Re-al-time PCR. The results showed that in comparing with the group immunized with G-H+pcD-NA3.1, the group communication with pcDNA3.1-pBoIL-2 induced significantly humoral and cellular immune responses. The results of this study indicate that the co-inoculation of G-H loop peptide with rBoIL-2 (as adjuvant) showed good immune effect.关键词
IL-2/FMDV/G-H环/多肽Key words
IL-2/ FMDV/ G-H loop/ peptide分类
农业科技引用本文复制引用
葛兰云,李勐,李爽,刘思莹,徐宗香,张润祥,高明春,马波,王君伟..牛IL-2重组质粒对口蹄疫病毒G-H环多肽免疫小鼠的佐剂效应[J].畜牧兽医学报,2009,40(11):1651-1657,7.基金项目
国家科技支撑计划课题(2006BAD06A17-08) （2006BAD06A17-08）
黑龙江省十一五重大科技项目(GA06B202) （GA06B202）
国家奶牛技术体系 （）
