畜牧兽医学报2009，Vol.40Issue(11)：1662-1668,7.
甘肃合作猪白细胞抗原经典Ⅱ类分子DR基因克隆及生物信息学分析
Molecular Cloning and Bioinformatics Analysis of Swine leukocyte Antigen Class Ⅱ DR Genes in Gansu Hezuo Pigs
摘要
Abstract
Class Ⅱ DRA and DRB genes of swine leukocyte antigen (SLA) of Gansu Hezuo pigs were cloned, and their characteristics and polymorphism were analyzed to explore immunological basic parameters for xenotransplantation from pigs to humans. SLA-DRA and SLA-DRB genes of Hezuo pigs were cloned by RT-PCR, sequenced and submited to Genbank (accession numbers: FJ905823 and FJ9058258). Analysis results through BLAST in NCBI and related software in Ex-PASy suggested that this kind of inbred strain of pig would be the animal model of choice for the porcine-human xenotransplantation after modification with gene engineering methods.关键词
猪白细胞抗原/合作猪/RT-PCR/生物信息学Key words
swine leukocyte antigen (SLA)/ Hezuo pig/ RT-PCR/ bioinformatics分类
农业科技引用本文复制引用
莫斯科,房永祥,冯海燕,景志忠..甘肃合作猪白细胞抗原经典Ⅱ类分子DR基因克隆及生物信息学分析[J].畜牧兽医学报,2009,40(11):1662-1668,7.基金项目
国家高新技术研究发展计划(863)项目(2006AA10A203) （863）
国家自然基金项目(30871884) （30871884）
转基因生物新品种培育重大专项(2009ZX08008-010B) （2009ZX08008-010B）
