云南农业大学学报2009,Vol.24Issue(5):654-660,7.
云南屏边大围山微型鸡遗传多样性
Genetic Diversity of Daweishan Mini Chickens Based on Microsatellite Markers
摘要
Abstract
Daweishan Mini chicken is unique chicken breed in China, which distributes in the tropic and sub-tropic zone in Pingbian county of Yunnan Province. In order to assess its genetic variability and population structure further,33 chicken-specific microsatellite loci were analyzed for a total of 30 individuals from small remote villages by using polyacrylaminde gel electrophoresis. A total of 65 alleles were detected across the 33 loci assayed. The number of alleles varied from 1 to 3,giving a mean number of 1.9697 alleles per locus.The number of effective alleles ranged from 1.00 to 2.63 and the mean of number of effective alleles was 1.6846 for per locus. The average heterozygosity and polymorphism information content of 33 microsatellite loci were 0.1737 and 0.3279. The results indicate that Daweishan Mini chicken has low heterozygosity and polymorphism information content and high homozygosity. They are a containing primitive chicken breed without hybrid with other breed and showed high genetic consistency.关键词
大围山微型鸡/微卫星标记/遗传变异/多态信息含量/杂合度Key words
Daweishan Mini chicken;microsatellite markers;genetic diversity/polymorphism information content;heterozygosity分类
农业科技引用本文复制引用
贾俊静,文生萍,熊保良,马振峰,苗永旺,张曦,李琦华,曹振辉,葛长荣..云南屏边大围山微型鸡遗传多样性[J].云南农业大学学报,2009,24(5):654-660,7.基金项目
云南省中青年学术带头人后备人才项目(2008PY043) (2008PY043)
教育部国家留学基金委项目 ()
云南农业大学博士启动经费资助. ()