应用化学2009，Vol.26Issue(10)：1144-1148,5.
毒死蜱分子印迹聚合物微球的制备及其结合特性
Synthesis and Characterization of Molecularly Imprinted Polymer Microspheres for Recognition of Chlorpyrifos
摘要
Abstract
Molecularly imprinted polymeric microspheres(MIPMs) were prepared by a one-step swelling polymerization method in an aqueous system with polystyrene microspheres obtained by emulsifer-free polyme-rization as seeds and Chlorpyrifos as template molecules. The selective binding characteristics of the MIPMs were evaluated by ultraviolet spectrometry. The infrared spectrum(IR) of the polymer further indicated that there were some functional groups in the moleculary imprinted polymer which could interact with the template. Subsequent morphological characterization of the MIPMs by means of scanning electron microscopy(SEM) shows that the diameters were between 0.5～3 μm(2～3 μm for UMIPMs), and surface rugosity and high porosity facilitated the approaching of the template to the binding sites, so as to obtain high load, affinity and selectivity for the template molecules. Scatchard analysis suggests that during MIPMs recognition of Chlorpyrifos, there were two classes of binding sites. The calculated dissociation constant K_(D1) and apparent maximum number B_(max1) of binding sites with high affinity were 0.526 mmol/L and 35.91 μmol/g respectively, while K_(D2) and B_(max2) of binding sites with low affinity were 2.19 mmol/L and 83.87 μmol/g.关键词
分子印迹聚合物微球/单步溶胀聚合/毒死蜱/结合位点Key words
molecularly imprinted polymeric microsphere/single-step swelling polymerization/chlorpyrifos/binding site分类
化学化工引用本文复制引用
淮路枫,杨明,刘骏,胡娟..毒死蜱分子印迹聚合物微球的制备及其结合特性[J].应用化学,2009,26(10):1144-1148,5.基金项目
湖北省自然科学基金(2004ABA095)、武汉工业学院创新基金(2007cx005)资助项目 （2004ABA095）
