针刺研究2009，Vol.34Issue(3)：152-158,7.
电针对β-淀粉样前体蛋白转基因小鼠行为学及其淀粉样前体蛋白、β淀粉样蛋白及胆碱乙酰转移酶水平的影响
Effect of Electroacupuncture on the Behavior and the Expression of Amyloid β-protein, Amyloid Precursor Protein and ChAT in APP 695 V 717 I Transgenic Mice
摘要
Abstract
Objective To observe the effect of electroacupuncture (EA) on the expression of amyloid precursor protein (APP) and amyloid β-protein (Aβ) in hippocampal CA 1 area and cerebral cortex in APP 695 V 717 I transgenic mice, so as to investigate its underlying mechanism in improving Alzheimer's disease (AD). Methods APP 695 V 717 I transgenic mice were randomly divided into model and EA groups, with 6 cases in each. The other 6 negatively-transgenic mice (C 57 BL/6 J) were used as the normal control group. EA (2 Hz/100 Hz, 3 mA) was applied to "Baihui" (GV 20) and "Yongquan" (KI 1) for 15 min, once every other day for 3 months. The learning-memory ability of mice was measured by Y-type maze test and the expression levels of APP, Aβ, and choline acetyltransferase (ChAT) in the cerebral cotex and hippocampal CA 1 area were detected by im-munohistochemistry. Results In comparison with control group, the times of training for gaining correct reaction and the expression levels of Aβ and APP in both cerebral cortex and hippocampal CA 1 area in model group increased significantly (P<0. 05, P<0. 01), while the expression level of ChAT of cerebral cortex in model group decreased considerably (P<0. 05). Compared to model group, the times of training for gaining correct reaction and the expression levels of APP in both cerebral cortex and hippocampal CA 1 area, and Aβ in CA 1 area of EA group lowered remarkably (P<0. 05), while those of ChAT in cerebral cortex and hippocampal CA 1 area of EA group increased evidently (P<0. 05, P<0. 01). Conclusion Electroacupuncture can improve the learning-memory capacity of the APP transgenic mice, which may be related to its effects in increasing the production of ace-tylcholine and lowering the levels of APP and Aβ in the brain.关键词
阿尔茨海默病/APP转基因小鼠/电针/学习记忆/大脑皮层/海马CA 1区Key words
Alzheimer's disease/ APP transgenic mice/ Electroacupuncture/ Learning-memory/ Cerebral cortex/ Hippocampal CA 1 area分类
医药卫生引用本文复制引用
薛卫国,张忠,白丽敏,许红,吴海霞..电针对β-淀粉样前体蛋白转基因小鼠行为学及其淀粉样前体蛋白、β淀粉样蛋白及胆碱乙酰转移酶水平的影响[J].针刺研究,2009,34(3):152-158,7.基金项目
高等学校博士学科点专项科研基金资助(No.20060026015) （No.20060026015）
