针刺研究2009，Vol.34Issue(3)：175-179,5.
电针对抑郁大鼠行为学、海马生长抑素及其mRNA表达的影响
Influence of Electroacupuncture on Hippocampal Somatostatin (SS) and SS mRNA Expression in Chronic Stress-induced Depression Rats
朱艺 1白春艳 1卓廉士 1冯雯琪1
作者信息
- 1. 重庆医科大学中医药学院,重庆,401331
- 折叠
摘要
Abstract
Objective To study the underlying mechanism of electroacupuncture (EA) in relieving depression. Methods A total of 30 Wistar rats were randomly divided into normal control group (n = 10), model group (n = 10) and EA group (n = 10). Depression model was established by lonely raising and chronic unpredictable mild stress for 21 days. EA (2 Hz, 1 mA) was applied to "Baihui" (GV 20) and "Sanyinjiao" (SP 6) for 20 min, once daily for 14 days. The expression (option density) of hippocampal somatostatin (SS) and SS mRNA was detected by using immunohistochemistry and reversed transcription-polymerase chain reaction (RT-PCR) techniques respectively. Results Compared to normal control group, the crossing movement and rearing movement times of open-field test, and sucrose consumption in model group decreased significantly (P<0. 01), and hippocampal SS and SS mRNA expressions were downregulated remarkably (P<0. 05). Compared to model group, the crossing movement and rearing movement times in EA group increased considerably (P<0. 01), and hippocampal SS and SS mRNA expressions were upregulated remarkably (P<0. 05). No significant difference was found between EA and normal control groups in SS and SS mRNA expression. Conclusion EA can effectively improve depression rats' behavior activity and upregu-late hippocampal SS and SS mRNA expression.关键词
抑郁症/电针/海马/生长抑素/免疫反应活性/基因表达Key words
Depression/ Electroacupuncture/ Hippocampus/ Somatostatin (SS) immunoreaction activity/ SS mRNA expression分类
医药卫生引用本文复制引用
朱艺,白春艳,卓廉士,冯雯琪..电针对抑郁大鼠行为学、海马生长抑素及其mRNA表达的影响[J].针刺研究,2009,34(3):175-179,5.
