针刺研究2009，Vol.34Issue(4)：248-251,4.
针刺颈夹脊穴对大鼠退变颈椎间盘细胞外基质金属蛋白酶1、3和金属蛋白酶抑制因子1的影响
Effects of Electroacupuncture at Jiaji(EX-B 2)on Extracellular Matrix MMP-1,MMP-3 and TIMP-1 Levels in the Degenerated Cervical Intervertebral Disc in Rats
摘要
Abstract
Objective To observe the effect of electroacupuncture (EA) of Jiaji (EX-B 2) on the contents of extracellu-lar matrix metalloproteinase (MMP)-I, MMP-3 and tissue inhibitor of matrix metalloproteinase (TIMP)-1 in the cervical interverte-bral disc (CIVD) tissue in rats with CIVD degeneration so as to explore its mechanism in relieving intervertebral disc degeneration. Methods SD rats were randomized into control (n = 15), model (n = 14), EA (n = 13) and medication (n = 14) groups. CIVD model was established according to Wang's and Shi's~([3-4]) method. EA (10/20 Hz, 1-3 mA) was applied to Jiaji (EX-B 2) for 10 min, once daily for 30 days. The rats of medication group were fed with Fenbid (15 mg/kg/d) and Jingfukang (0. 5 g/kg/d) for 30 days. Extracellular matrix MMP-1, MMP-3 and TIMP-1 levels of the intervertebral disc tissue (C_(4-6)) were detected by ELISA. Results The contents of MMP-1 and MMP-3 in model group were increased significantly compared with con-trol group (P<0. 05), while TIMP-1 in model group was markedly lower than that of control group (P<0. 05). Compared with model group, both MMP-1 and MMP-3 levels in EA group and MMP-1 in medication group were reduced considerably (P<0. 05), while TIMP-1 of EA group increased significantly (P<0. 05). No significant differences were found between medication and model groups in MMP-3 and TIMP-1 levels (P>0. 05). Under optical microscope, the injured degrees of the fibrous ring and nucleus pul-posus, and the outline of the intervertebral disc of both EA and medication groups were evidently lighter than those of model group, but the degeneration severity of the intervertebral disc of EA group was lighter than that of medication group. Conclusion EA of Jiaji (EX-B 2) can effectively relieve the degeneration degree of cervical intervertebral disc in the rat, which is probably re-lated to its effect in regulating extracellular matrix system.关键词
颈椎间盘退变/电针/颈夹脊穴/细胞外基质MMP-1、MMP-3、TIMP-1Key words
Cervicalintervertebral disc degeneration/ Electroacupuncture,-Jiaji (EX-B 2),-Extracellular matrix MMP-1, MMP-3, TIMP-1分类
医药卫生引用本文复制引用
王学新,李永春,马晓芃,韩兆诚..针刺颈夹脊穴对大鼠退变颈椎间盘细胞外基质金属蛋白酶1、3和金属蛋白酶抑制因子1的影响[J].针刺研究,2009,34(4):248-251,4.基金项目
山东省中医药科技发展计划项目(No.2005239)、烟台市科学技术发展计划(No.2006131-6) （No.2005239）
