针刺研究2009，Vol.34Issue(5)：291-296,6.
用于针刺穴位研究的结缔组织片模型
Skin Slice Used as a Model for Investigating Acupuncture Effects
摘要
Abstract
Objective To establish an acupoint-connective tissue model for studying the mechanism of acupuncture by using in vitro patch clamp technique in the rat skin slices. Methods The local connective tissue under the corium of "Housanli" (ST 36) area from SD rat was acutely and bluntly separated and fixed in a chamber filled with artificial incubation solution. Mast cells in the prepared connective tissue slice were labeled by toluidine blue (TB) or neutral red (NR). The whole-cell current of mast cells responding to pressure stimulation applied through a patch pipette was recorded in rat slices derived from acupoint ST 36 area by using in vitro patch-clamp technique. Results 1) After staining with TB and NR, the labeled mast cells were found to distribute in the extracellular matrix of the connective tissue samples, and their degranulation phenomenon could be seen clearly. 2) The whole-cell current of mast cells in response to mechanical stress stimulation was successfully recorded in the connective tissue slices of the rat acupoint ST 36 area. The cellular membrane currents increased evidently when pressure gradients of -30, -60 or -90 cmH_20 were applied to the recorded mast cells. Conclusion The connective tissue slice from the rat ST 36 area may be used as a model for investigating the peripheral mechanism of acupuncture by combining the microtechniques and electrophysiological techniques. The results obtained in this model prove for the first time by electrophysiology that the mast cells in the connective tissue are probably involved in the transduction process of the mechanical signal from acupuncture stimulation. This new model provides a base for investigating the characters of the cells, collagen fibers, proteoglycans, etc. and their interactions in the acupoint connective tissue in the future.关键词
针刺/结缔组织片/肥大细胞/机械激活性/膜片钳Key words
Acupuncture/Connective tissue slice/Mast cells/Mechano-sensitive current/Patch clamp分类
生物科学引用本文复制引用
汪丽娜,Wolfgang Schwarz,顾全保,丁光宏..用于针刺穴位研究的结缔组织片模型[J].针刺研究,2009,34(5):291-296,6.基金项目
上海市重点学科建设资助项目(B112、S30340、T0302),上海市科技发展基金(No.05 DZ 19745、06 DZ 19732、064319053、07DZ 19722和07 DZ19733)和国家重点基础研究发展计划(973项目,No.2005 CB 523306)资助 （B112、S30340、T0302）
