蔡玉颖 1刘志顺 1王顺 2王奇峰 2崔晓梅 2曲龙2
作者信息
- 1. 中国中医科学院广安门医院针灸科,北京,100053
- 2. 黑龙江省中医研究院针灸科,哈尔滨,150036
- 折叠
摘要
Abstract
Objective To observe the effect of electroacupuncture (EA) on the related hormones of the hypothalamuspituitary-adrenal (HPA) axis in rats with cerebral ischemia-reperfusion injury (CI-RI). Methods Ninety Wistar rats were randomly divided into normal control, sham-operation (sham), model, EA acupoint and EA non-acupoint groups. CI-RI model was established by using modified middle cerebral artery occlusion (MCAO) and reperfusion. These rats were further divided into 1 day (d), 3d and 7 d subgroups, with 6 cases in each. EA (1 mA, 2 Hz/30 Hz) was applied to acupoint "Zusanli" (ST 36) and "Quchi" (LI 11), and non-acupoints (5 mm lateral to ST 36 and 5 mm apart from LI 11 on the radial side respectively) for 30 min,once daily for 1 d, 3 d and 7 d separately. Serum cortisole (CORT) content was assayed by enzyme linked immunosorbent assay (ELISA), and the expression of hypothalamic adrenocorticotropic releasing factor (CRF) mRNA and glucocorticoid receptor (GR) mRNA, and pituitary adrenocorticotrophic hormone (ACTH) mRNA was assayed by reverse transcription polymerase chain reaction (RT-PCR) method. Results Compared with normal and sham groups, serum CORT levels of model and EA non-acupoint groups on the 1~(st), 3~(rd) and 7~(th) day increased significantly (P<0. 05), while compared with model groups, serum CORT level of EA acupoint groups decreased apparently (P<0. 05). Compared with normal and sham groups, hypothalamic CRF mRNA and pituitary ACTH mRNA expression of model groups were upregulated significantly on the 1~(st), 3~(rd) and 7~(th) d after CI-RI (P<0. 01, P<0. 05), and hypothalamic GR mRNA expression of model groups were obviously downregulated (P<0. 01, P<0. 05). In comparison with model groups, hypothalamic CRF mRNA and pituitary ACTH mRNA expression of EA acupoint groups at the 3 time-points, and their expression of EA non-acupoint groups on the 1~(st) and 3~(rd) day were remarkably downregulated (P<0. 01, P<0. 05), and hypothalamic GR mRNA expression of EA acupoint groups was upregulated significantly at the 3 time-points (P<0. 01, P<0. 05). Comparison between EA acupoint and EA non-acupoint groups showed that the effect of EA acupoint groups was obviously superior to that of EA non-acupoint groups in downregulating CRF mRNA and ACTH mRNA expression and in upregulating GR mRNA expression at the 3 time-points (P<0. 01, P<0. 05). Conclusion EA of meridian-acupoints can effectively downregulate serum CORT content, hypothalamic CRF mRNA and pituitary ACTH mRNA expression and upregulate hypothalamic GR mRNA expression in CI-RI rats, which may contribute to its effect in relieving CI-RI.关键词
脑缺血再灌注/电针/经穴/下丘脑-垂体-肾上腺轴/激素Key words
Cerebral ischemia reperfusion injury/ Electroacupuncture/ Meridian-acupoints/ HPAaxis/ Serum cortisole/ Hypothalamic CRF mRNA and GR mRNA/ Pituitary ACTH mRNA分类
生物科学
