针刺研究2009，Vol.34Issue(5)：309-314,6.
电针对转基因阿尔茨海默病小鼠海马CA1区超微结构的影响
Effect of Electroacupuncture on the Behavior and Hippocampal Ultrastructure in APP 695 V 717 I Transgenic Mice
摘要
Abstract
Objective To investigate the ultrastructural basis underlying electroacupuncture (EA) induced improvement of Alzheimer disease (AD) in transgenic mice. Methods Twelve APP 695 V 717 I transgenic mice were randomly divided into model group and EA group; and other 6 negative transgenic mice (C 57 BL/6 J) were made up of normal control group. After 3 months treatment by EA (15 min per other day, 2 Hz/100 Hz, 3-4 mA) applied to "Baihui" (GV 20) and "Yongquan" (Kl 1), the learning and memory ability of mice was measured by Lashley M water maze test, and the ultrastructural changes of hippocampal CA 1 region was observed by electronic microscopy. Results The swimming escape latency and the number of navigating errors (dead-end forward swimming) in model group were significantly longer and more than those in normal control group (P<0. 05); and those in EA group were considerably shorter and fewer than those in model group (P<0. 05), suggesting an improvement of learning-memory ability after EA. Comparison of the ultrastructure of the neurons in the hippocampal CA 1 region showed swelling of the mitochondria, broken or disappearance of the mitochondrial cristae, degeneration of the synapses, breakage and vague outline of the basement membrane of the blood capillaries in mice of model group; and basically distinct outline of the mitochondrial cristae and microvessels, and more synaptic vesicles in EA group. Conclusion EA may effectively improve the learning-memory capacity of the APP transgenic AD mice and alleviate the pathological changes of neurons of the hippocampal CA 1 region, which may be one of the mechanisms underlying the improvement of AD by EA.关键词
电针/APP转基因小鼠/阿尔茨海默病Key words
Electroacupuncture/ APP 695 V 717 I transgenic mice/ Alzheimer disease分类
医药卫生引用本文复制引用
薛卫国,葛桂玲,张忠,许红,白丽敏..电针对转基因阿尔茨海默病小鼠海马CA1区超微结构的影响[J].针刺研究,2009,34(5):309-314,6.基金项目
高等学校博士学科点专项科研基金资助(No.20060026015) （No.20060026015）
