针刺研究2009，Vol.34Issue(1)：27-30,4.
针刺"内关""中脘""足三里"对背侧网状亚核神经元放电的影响
Effects of Electroacupuncture of "Neiguan" (PC 6),"Zhongwan" (CV 12) and "Zusanli" (ST 36)on Electrical Activities of Neurons in Subnucleus Reticularis Dorsalis in Rats
摘要
Abstract
Objective To compare the effects of electroacupuncture (EA) stimulation of "Gastric Problem Formula" ["Neiguan" (PC 6), "Zhongwan" (CV 12) and "Zusanli" (ST 36)] on electrical activities of neurons of medullary subnucleus reticularis dorsalis (SRD) of the rat. Methods Experiments were performed on SD rats anesthetized by 10% urethane (1.0 -1. 5 g/kg). Under artificial ventilation and immobilization (2% gallamine triethiodide, 5 ml/kg), extracelluar discharges were recorded by glass micropipettes inserted into SRD of the medulla (Caudal to the Obex: - 1. 0-2. 0 mm, L/R: 0. 5- 1. 5 mm, H: 0. 6- 1. 5 mm below the medullary surface). Following getting stable recordings, the acupuncture needle inserted in "Neiguan" (PC 6) was manipulated moderately (twisting range: 120°, frequency: 80 times/min) for 30 s. The same manipulation was performed at "Zhongwan" (CV 12) and "Zusanli" (ST 36) separately. Results After moderate acupuncture stimulation, in the recorded 13 SRD neurons, the discharges were increased from (1. 67±0. 35) spikes/s to (17. 55± 1. 40) spikes/s for "Neiguan" (PC 6), from (2. 35 ±0. 43) spikes/s to (16. 96 ±1. 83) spikes/s for "Zhongwan" (CV 12), and from (1. 83±0. 37) spikes/s to (17. 39 ± 1. 58) spikes/s for "Zusanli" (ST 36). No apparent differences were found among 3 acupoints in their responses to manual manipulation. Conclusion Moderate acupuncture stimulation of "Neiguan" (PC 6), "Zhongwan" (CV 12) and "Zusanli" (ST 36) can effectively activate spontaneous discharges of SRD neurons in the rat.关键词
背侧网状亚核/神经元放电/针刺/内关/中脘/足三里Key words
Medullary SRD/ Neuron discharge/ Acupuncture/ PC6/ CV12/ ST36分类
生物科学引用本文复制引用
冀来喜,闫丽萍,王海军,李亮,何伟,贲卉,朱兵..针刺"内关""中脘""足三里"对背侧网状亚核神经元放电的影响[J].针刺研究,2009,34(1):27-30,4.基金项目
国家自然科学基金资助项目(30572422) （30572422）
