针刺研究2009，Vol.34Issue(1)：48-51,4.
嗅三针对老年痴呆大鼠学习记忆功能及海马胆碱乙酰化酶、乙酰胆碱酯酶活性的影响
Effect of "Xiusanzhen" on Learning-memory Ability and Hippocampal ChAT and AChE Activity in Alzheimer Disease Rats
摘要
Abstract
Objective To explore the effect of "Xiusanzhen" [bilateral "Yingxiang" (LI 20) + "Yintang" (EX-HN 3)] on learning-memory ability and choline acetyl transferase (ChAT) and acetyl cholinesterase (AChE) activity of hippocampus tissue in Alzheimer Disease (AD) rats. Methods A total of 40 Sprague Dawley rats were randomly divided into control, AD model, AD + olfactory-bulb lesion (OBL, induced by electrocoagulation method) + EA and "Xiusanzhen" (AD+ EA) groups. AD model was established by 4-vessel occlusion. Morris water maze tests were conducted for evaluating the rat's learning-memory ability. The activity of ChAT and AChE of hippocampus tissue was detected by colorimetric method. Results In comparison with control group, the average escape latency (AEL) prolonged significantly and the target-platform crossing times decreased remarkably in AD model group (P<0. 01). In comparison with AD model group, the AEL shortened and the target-platform crossing times increased remarkably in AD+EA group (P<0. 01). No significant differences were found between AD model and AD + OBL+EA groups in AEL and the target-platform crossing times (P>0. 05). The ChAT and AChE activity of hippocampus in AD model group was significantly lower than that of control group (P<0. 05), while that of ChAT and AChE in AD+ EA group was significantly higher than that in AD model group (P<0. 05). No significant difference was found between AD model and AD + OBL+ EA groups in ChAT and AChE activity of hippocampus (P>0. 05). Conclusion EA of "Xiusanzhen" can markedly raise the learning-memory ability in AD rats, which may be related to its effect in increasing the ChAT and AChE activity of hippocampus in AD rats. Its therapeutical effect also depends on the intact of the olfactory pathway.关键词
老年性痴呆/嗅球损毁/电针/嗅三针/学习记忆/海马/ChAT/AChEKey words
Alzheimer Disease/ Olfactory bulb/ Electroacupuncture/ "Xiusanzhen"/ Learning-memory/ Hippo-campus/ ChAT and AChE activity分类
医药卫生引用本文复制引用
刘智斌,牛文民,杨晓航,牛晓梅,王渊..嗅三针对老年痴呆大鼠学习记忆功能及海马胆碱乙酰化酶、乙酰胆碱酯酶活性的影响[J].针刺研究,2009,34(1):48-51,4.基金项目
陕西省科学技术研究发展计划项目[N0.2007K13-03(17)] （17）
