中国病理生理杂志2009，Vol.25Issue(11)：2192-2196,5.
银杏内酯B对体外培养的视网膜神经细胞内钙离子浓度和线粒体功能的影响
Effects of ginkgolide B on [ Ca~(2+) ]_i and mitochondrial function of cultured rat retinal neurons in vitro
摘要
Abstract
AIM: To observe the effect of ginkgolide B (CB) on the intracellular calcium ion concentration ( [ Ca~(2+) ]_i) and mitochondrial function of cultured rat retinal neurons in vitro. METHODS: in vitro primary culture of rat retinal neurons was used in the experiment. The apoptosis model of glutamate - induced retinal neurons was established and co - cultured with ginkgolide B. The [ Ca~(2+) ]_i and mitochondrial membrane potential of the retinal neurons were detected by laser scanning confocal microscope. RESULTS: Glutamate decreased the survival rate of retinal neurons, increased the apoptosis and the [ Ca~(2+) ]_i, lowered the mitochondrial membrane potential. The [ Ca~(2+) ]_i was clearly diminished and the mitochondrial membrane potential was significantly increased with the GB intervention, and the apoptosis decreased significantly. CONCLUSION: GB protects retinal neurons from glutamate induced neurotoxicity. The effect of GB on retinal neurons might be due to its ability to decrease the [Ca~(2+) ]_i and increase mitochondrial membrane potential.关键词
银杏内酯B/谷氨酸/细胞内钙离子浓度/线粒体膜电位/视网膜Key words
Ginkgolide B/ Glutamic acid/ Intracellular calcium ion concentration/ Mitochondrial membrane potential/ Retina分类
医药卫生引用本文复制引用
孟晶,丁小燕,朱晓波,林少芬,郭梦翔,唐仕波..银杏内酯B对体外培养的视网膜神经细胞内钙离子浓度和线粒体功能的影响[J].中国病理生理杂志,2009,25(11):2192-2196,5.基金项目
广东省科技计划基金资助项目(No.A050100029) （No.A050100029）
广东省医学科研基金资助项目(No.B2009125) （No.B2009125）
