中国当代儿科杂志2009，Vol.11Issue(11)：897-900,4.
91例EB病毒相关疾病儿童血浆EB病毒DNA的检测
Determination of plasma EBV DNA in 91 children with EBV-associated diseases
摘要
Abstract
Objective To determine the plasma level of Epstein-Barr virus ( EBV) DNA in children with EBV-associated diseases, and to investigate the dynamic changes of EBV DNA level after initial infection as well as the relationship between EBV-DNA level and the diseases severity. Methods The subjects consisted of 73 children with primary EBV infection (infectious mononucleosis, pneumonia,etc. ) and 18 children with severe EBV-associated diseases (chronic active EBV infection, hemophagocytic lymphohistiocytosis, etc. ). The plasma EBV DNA level was detected by a real-time PCR assay. Results The plasma EBV DNA level decreased with the infection time in children with primary EBV infection. Two weeks after infection, plasma EBV DNA was almost undetectable. The positive rate of plasma EBV DNA in children with severe EBV-associated diseases increased significantly when compared with that in children with primary EBV infection (89% vs 16% ; P < 0.05). Conclusions The level of EBV replication may be reduced with the infection time. Dynamic determination of blood EBV DNA is useful for the evaluation of disease severity in children with EBV infection.关键词
EB病毒/慢性活动性EB病毒感染/噬血细胞性淋巴组织细胞增生症/传染性单核细胞增多症/儿童Key words
Epstein-Barr virus/ Chronic active Epstein-Barr virus infection/ Hemophagocytic lymphohistiocytosis/ Infectious mononucleosis/ Child分类
医药卫生引用本文复制引用
段红梅,姚瑶,谢正德,闫静,胡英慧,幺远,周玲,申昆玲..91例EB病毒相关疾病儿童血浆EB病毒DNA的检测[J].中国当代儿科杂志,2009,11(11):897-900,4.基金项目
北京市科委基金(z0005190042511)、北京市教委基金(KM200710025023)及北京市自然科学基金(702026)资助. （z0005190042511）
