中国当代儿科杂志2009，Vol.11Issue(11)：923-926,4.
产前补充牛磺酸对宫内生长受限胎鼠脑组织PKA-CREB通路及GDNF mRNA表达的影响
Effects of prenatal taurine on mRNA expression of PKA-CREB signal pathway and glial cell line-derived neurotrophic factor in fetal rat brains of intrauterine growth re-striction
摘要
Abstract
Objective This study examined the effects of prenatal application of taurine on mRNA expression of protein kinase A-cAMP response element binding protein ( PKA-CREB) signal pathway and glial cell line-derived neurotrophic factor (GDNF) in fetal rat brains of intrauterine growth restriction (IUGR). Methods Pregnant rats were randomly divided into4 groups; normal control, IUGR model, low-dose (100 mg/kg · d) and high-dose (300 mg/kg · d) taurine treatment IUGR (n = 5 each). IUGR was induced by food restriction throughout pregnancy. PKA, CREB and GDNF mRNA expression in brains of newborn rats was detected by reverse transcription-polymerase chain reaction (RT-PCR). Results PICA, CREB and GDNF mRNA expression in the IUGR model group was significantly higher than that in the normal control group (P<0.05). Compared with the IUGR model group, mRNA expression of PKA and CREB in both the low-dose and high-dose taurine treatment groups increased significantly (P < 0. 05); GDNF mRNA expression in the high-dose taurine treatment group also increased significantly (P < 0. 01 ). Conclusions Taurine can increase mRNA expression of PKA, CREB and GDNF in fetal rat brains of IUGR. This suggests that prenatal application of taurine may increase neurogenesis of the central nervous system and endogenous secretion of neurotrophic factors, thus providing neuroprotective effects.关键词
宫内生长受限/牛磺酸/cAMP反应元件结合蛋白/胶质细胞源性神经营养因子/胎鼠Key words
Intrauterine growth restriction/ Taurine/ cAMP response element binding protein/ Glial cell line-derived neurotrophic factor/ Fetal rats分类
医药卫生引用本文复制引用
陈慧,李坚,刘敬,刘丽,刘娜,宋一志..产前补充牛磺酸对宫内生长受限胎鼠脑组织PKA-CREB通路及GDNF mRNA表达的影响[J].中国当代儿科杂志,2009,11(11):923-926,4.基金项目
首都医科大学基础-临床合作基金资助项目(2007JL53). （2007JL53）
