首页|期刊导航|中国海洋湖沼学报（英文版）|Effect of water temperature on digestive enzyme activity and gut mass in sea cucumber Apostichopus japonicus (Selenka), with special reference to aestivation
中国海洋湖沼学报（英文版）2009，Vol.27Issue(4)：714-722,9.DOI:10.1007/s00343-009-9202-3
Effect of water temperature on digestive enzyme activity and gut mass in sea cucumber Apostichopus japonicus (Selenka), with special reference to aestivation
Effect of water temperature on digestive enzyme activity and gut mass in sea cucumber Apostichopus japonicus (Selenka), with special reference to aestivation
摘要
Abstract
The effect of water temperature on gut mass and digestive enzyme activity in sea cucumber Apostichopus japonicus, including relative gut mass (RGM), amylase, lipase, pepsin and trypsin activities were studied at temperatures of 7, 14, 21, and 28°C over a period of 40 days. Results show that RGM significantly decreased after 40 days at 21°C and markedly decreased over the whole experiment period at 28°C; however, no significant effect of duration was observed at 7 or 14°C. At 14°C, trypsin activity significantly decreased over 10 and 20 days, then increased; amylase and trypsin activity significantly decreased after 40 days at 28°C. However, no significant effect of duration was found on amylase, pepsin or trypsin activities in the other temperature treatment groups. At 28°C, lipase activity peaked in 20 days and then markedly decreased to a minimum at the end of the experiment. On the other hand, pepsin activity at 28°C continuously increased over the whole experimental period. Principle component analysis showed that sea cucumbers on day 40 in the 21°C group and in the previous 20 days in the 28°C group were in the prophase of aestivation. At 28°C, sea cucumbers aestivated at 30-40 days after the start of the experiment. It is concluded that the effect of temperature on the digestion of A. japonicus is comparatively weak within a specific range of water temperatures and aestivation behavior is accompanied by significant changes in RGM and digestive enzyme activities.关键词
digestive enzyme activity/ relative gut mass (RGM)/ aestivation/ water temperature/ Apostichopus japonicusKey words
digestive enzyme activity/ relative gut mass (RGM)/ aestivation/ water temperature/ Apostichopus japonicus分类
海洋科学引用本文复制引用
GAO Fei,YANG Hongsheng,XU Qiang,WANG Fangyu,LIU Guangbin..Effect of water temperature on digestive enzyme activity and gut mass in sea cucumber Apostichopus japonicus (Selenka), with special reference to aestivation[J].中国海洋湖沼学报（英文版）,2009,27(4):714-722,9.基金项目
Supported by the Science Fund for Creative Research Groups (No.40821004), National Natural Science Foundation of China (No. 40576073), Breeding Project of Shandong Province (China), National Key Technology Research and Development Program of China (No. 2006BAD09A02), and the National High Technology Research and Development Program of China (863 Program) (No.2006AA100304 /2006AA10A411) （No.40821004）
