首页|期刊导航|中国海洋湖沼学报（英文版）|The selection of alkaline protease-producing yeasts from marine environments and evaluation of their bioactive peptide production
中国海洋湖沼学报（英文版）2009，Vol.27Issue(4)：753-761,9.DOI:10.1007/s00343-009-9198-8
The selection of alkaline protease-producing yeasts from marine environments and evaluation of their bioactive peptide production
The selection of alkaline protease-producing yeasts from marine environments and evaluation of their bioactive peptide production
摘要
Abstract
A total of 400 yeast strains from seawater, sediments, saltern mud, marine fish guts, and marine algae were obtained. The protease activity of the yeast cultures was estimated, after which four strains (HN3.11, N11b, YF04C and HN4.9) capable of secreting extracellular alkaline protease were isolated. The isolated strains were identified as Aureobasidium pullulans, Yarrowia lipolytica, Issatchenkia orientalis and Cryptococcus cf. aureus. The optimal pH of the protease activity produced by strains HN3.11, YF04C, and HN4.9 was 9.0, while that of the protease produced by strain N11b was 10.0. The optimal temperature for protease activity was 45°C for strains HN3.11, N11b, and YF04C, and 50°C for strain HN4.9. After digestion of shrimp (Penaeus vannamei) protein and spirulina (Arthospira platensis) protein with the four crude alkaline proteases, the filtrate from spirulina (Arthrospira platensis) powder digested by the crude alkaline protease of strain HN3.11 was found to have the highest antioxidant activity (61.4%) and the highest angiotensin I converting enzyme (ACE)-inhibitory activities (68.4%). The other filtrates had much lower antioxidant activity and ACE-inhibitory activities.关键词
marine yeasts/ alkaline protease/ yeast diversity/ phylogenetic analysis/ bioactive peptideKey words
marine yeasts/ alkaline protease/ yeast diversity/ phylogenetic analysis/ bioactive peptide分类
海洋科学引用本文复制引用
LI Jing,CHI Zhenming,WANG Xianghong,PENG Ying,CHI Zhe..The selection of alkaline protease-producing yeasts from marine environments and evaluation of their bioactive peptide production[J].中国海洋湖沼学报（英文版）,2009,27(4):753-761,9.基金项目
Supported by the High Technology Research and Development Program of China (863 Program) (No. 2006AA09Z403) and the National Science and Technology Infrastructure Program of China (No. 2005DKA21209) （863 Program）
