中国临床药理学与治疗学2009，Vol.14Issue(1)：37-41,5.
皖南尖吻蝮蛇毒提取物体外抗肿瘤活性的实验研究
Anticancer activity of extractive purified from Agkistrodon acutus Venom in vitro
摘要
Abstract
AIM: To purify snake venom metallo-proteinase x(SVMP-x)from Agkistrodon acutus Venom and investigate its anticancer activity in vitro. METHODS: The SVMP-x was separated from Agkistrodon acutus Venom by ion exchange using DEAE-Sepharose FF and SP-Sepharose FF and further purified by gel filtration through sephadex G75 columns. Different origins of tumor cell lines were cultured with different concentrations of SVMP-x. Vehicle and 5-FU were used as negative and positive control respectively. The in vitro anticancer activity of SVMP-x was determined by CCK-8 assay. RESULTS: SVMP-x was purifed from Agkistrodon acutus Venom. CCK-8 assay demonstrated that SVMP-x inhibited the proliferation of SGC7901, SW480, SH-SY5Y and B16 in a dose-dependent manner. The inhibition rates of SVMP-x to SGC7901 and B16 were significantly higher than that of 5-FU positive control. SVMP-x inhibited the proliferation of SGC7901 in a time-and dose-dependent manner. CONCLUSION: An anticancer component SVMP-x is successfully purified from Agkistrodon acutus Venom and SVMP-x inhibits the proliferation of a variety of tumor cell lines in vitro.关键词
尖吻蝮蛇/SVMP-x/纯化/抗肿瘤Key words
Agkistrodon acutus/SVMP-x/purification/anticancer分类
医药卫生引用本文复制引用
陈冬云,潘学兵,吉兆宁..皖南尖吻蝮蛇毒提取物体外抗肿瘤活性的实验研究[J].中国临床药理学与治疗学,2009,14(1):37-41,5.基金项目
安徽省教育厅自然科学基金项目(KJ2007A039) （KJ2007A039）
