中国临床药理学与治疗学2009，Vol.14Issue(1)：80-83,4.
HPLC-MS/MS法与MEIA法在监测器官移植患者他克莫司全血浓度中的比较
Comparison on two methods of HPLC-MS/MS and MEIA in monitoring the tacrolimus concentrations for organ transplantation patients
李鹏飞 1刘丽宏 1马萍 1丁春雷 1童卫杭1
作者信息
- 1. 中国人民解放军第二炮兵总医院药剂科,北京,100088
- 折叠
摘要
Abstract
AIM: To compare two methods of HPLC-MS/MS and MEIA to monitor the tacrolimus concentrations for organ transplantation patient.METHODS: After developing and validating the HPLC-MS/MS method to determine the concentrations of tacrolimus, the tacrolimus samples were quantitated by two methods of HPLC-MS/MS and MEIA, respectively.Evaluate two methods by statistical analysis of quantitative results.RESULTS: The mean concentration were(4.86 ±0.46) ng/mL by HPLC-MS/MS and ( 5.52 ± 0.43 ) ng/mL by MEIA.The coefficient correlation of two methods was 0.8771, and the two methods have good correlations in statistics.The mean concentration ratio was (90.3 ± 5.3)%.CONCLUSION: The HPLC-MS/MS method is a more precise method for to determining the effective concentration of tacrolimus, which is suitable for daily TDM.关键词
高效液相色谱-质谱联用法/微粒子捕捉酶免疫发光技术/器官移植/他克莫司Key words
HPLC-MS/MS/microparticle enzyme immunoassay/organ transplantation/tacrolimus分类
医药卫生引用本文复制引用
李鹏飞,刘丽宏,马萍,丁春雷,童卫杭..HPLC-MS/MS法与MEIA法在监测器官移植患者他克莫司全血浓度中的比较[J].中国临床药理学与治疗学,2009,14(1):80-83,4.
