中国临床药理学与治疗学2009，Vol.14Issue(3)：307-312,6.
液相色谱-串联电喷雾离子阱质谱法研究人尿中埃坡霉素D的主要代谢产物
Study on essential metabolites of Epothilone D in human urine by liquid chromatography-mass spectrometry
摘要
Abstract
AIM: To investigate the essential metabolites of Epothilone D in human urine. METHODS: The urine samples of six volunteers after inter-venous drop infusion of 125 mg/m~2 were collected within 0 - 72 h, the samples were purified and separated through C_(18) solid-phase extraction column and analyzed with liquid chromatography-mass spectrometry. The potential metabolites were analyzed through comparing the TIC and SIM chromatograms of the blank urine and sample urine, and the structure of the metabolites were identified through the correlation of the full scan MS~n spectra of Epothilone D and the metabolites. RE-SULTS: Eleven metabolites were found in human urine. There were three kinds of structure of metabolites, the metabolic ways of oxidation, hydrogenise, hydrolization and association reaction. CONCLUSION : The method was proved sensitively and selectively, and it is possible to supply a reliable reference for further study on the metabolites of Epothilones.关键词
高效液相色谱-串联质谱/ 埃坡霉素D/ SPE C_(18)固相萃取技术/ 代谢产物分析Key words
liquid chromatography-mass spectrometry/ epothilone D/ solid-phase extraction/ metabolite分类
医药卫生引用本文复制引用
张春玲,窦桂芳,朱晓霞,齐文渊,刘江林,孟志云,顾若兰,王延琳,吴卓娜,孙国祥..液相色谱-串联电喷雾离子阱质谱法研究人尿中埃坡霉素D的主要代谢产物[J].中国临床药理学与治疗学,2009,14(3):307-312,6.基金项目
国际科技合作重点项目计划(2005DFA30080) （2005DFA30080）
