中国临床药理学杂志2009，Vol.25Issue(6)：514-517,4.
红花注射液引起过敏反应的实验研究
Experimental study of anaphylaxis caused by Carthamus tinctorious(Safflower)injection in a guinea pig model
张颖 1张志伟 1朱青 2徐晓军1
作者信息
- 1. 北京大学基础医学院免疫学系,北京,100191
- 2. 北京市临床药学研究所,北京,100035
- 折叠
摘要
Abstract
Objective To separate and characterize the allergens in Carthamus tinctorious (Safflower) injection and identify related immunology characteristics. Methods Safflower injection was separated into several ingredients by chemical extraction, distillation and chromatography. Animal model of immediate hypersensitivity was established by sensitizing guinea pigs with peritoneal injection of Safflower and its ingredients, followed by intracardiac injection of the same ingredients in fortonight. ELISA was performed to detect the Safflower specific IgG antibody in human blood samples. RT-PCR was used to identified weather there is an stimulation effect of Safflower injection on macrophages of BalB/c mice. Results Anaphylaxis was induced by Safflower injection and its ingredients in guinea pigs model. However, Safflower specific IgG was not found in human blood sample, no significant stimulation effect on splenocytes and macrophages by Safflower and its ingredients was detected. Conclusion The material caused anaphylaxis in Safflower injection are more likely the liposoluble ingredients, the Safflower injection may be purified on it's physical/chemical characteristics.关键词
红花注射液/过敏反应Key words
Safflower injection/anaphylaxis分类
医药卫生引用本文复制引用
张颖,张志伟,朱青,徐晓军..红花注射液引起过敏反应的实验研究[J].中国临床药理学杂志,2009,25(6):514-517,4.
