中国临床药理学与治疗学2009，Vol.14Issue(5)：557-563,7.
当归芍药散治疗痛经的方药量效关系研究
Study on the dose-effect relationship of Danggui-Shaoyao-San's ingredients proportion in dysmenorrhea mice
摘要
Abstract
AIM: To analyze the influence of different combinations and proportions of Danggui-Shaoyao-San's ingredients in treating primary dysmen-orrhea. METHODS: The dysmenorrhea mouse model was established by oxytocin. The ingredient compati- bility was analyzed by orthogonal t design and the appropriate proportion relationship was researched by uniform design. The latent period and number of twisting body were recorded to evaluate the intensity of dysmenorrhea. The algomenorrhea effect was observed with the treatment of Danggui-Shaoyao-San or the combination of different ingredients in a prescription. RESULTS: The orthogonal t design experimental results showed the latent period was significantly prolonged by Paeonia lactiflora Pall. ( B) , Angelica sinensis Diels. ( A) and Ligusticum chuanxiong Hort. (C) , the number of twisting body was decreased by B. Poria cocos Wolf. (D), Alisma orientalis Juzep ( E) and Atractylodes macrocephala Koidz. (F) had no significant influence on the latent period and the number of twisting body. The uniform design results showed the indexes were fitted with regression equation, the data of each variable had mild surface effect, the proper proportion between A : B : C was 8.28 : 13.3 : 4.6 through surface analysis, and the above conclusion was proved. CON- CLUSION: The influnce on twisting body in algomen-orrhea mouse, of B was obvious, while A, C take the second place, D E, F played auxiliary function. The study gave strong support to the original formula to put B in a very important position, and it was speculated that slightly modulating the proportion of ingredients might bring better effects. The orthogonal t design and uniform design are valuable methods in studying multi-ingredient formulae of traditional Chinese medicine.关键词
当归芍药散/痛经/量效关系/正交t值法/均匀设计Key words
Danggui-Shaoyao-San/ dysmenor-rhea/ dose-effect relationship/ orthogonal t design/ uniform design分类
医药卫生引用本文复制引用
华永庆,谢海棠,段金廒,宿树兰,秦萍..当归芍药散治疗痛经的方药量效关系研究[J].中国临床药理学与治疗学,2009,14(5):557-563,7.基金项目
江苏省高校自然科学重大基础研究项目(06KJA36066) （06KJA36066）
江苏省方剂研究重点实验室开放课题(022021014002)资助 （022021014002）
