中国临床药理学与治疗学2009，Vol.14Issue(7)：766-769,4.
苦瓜蛋白逆转K562/AO2细胞多药耐药性的研究
Study on reversal of multidrug resistance in K562/AO2 cells by momordica
摘要
Abstract
To study the reverse and apopto-sis effects of momordica at non-cytotoxic concentration reversing the multidrug resistance (MDR) leukemia cell line K562/AO2. METHODS: The cytotoxic effect of momordica and the sensitivity to the K562/AO2 cell lines were determined by CCK-8 colorimetry. The ap-optosis rate of the K562/AO2 cell lines in control group and other groups were detected by flow cytometry. RE-SULTS: Momordica had definite cytotoxic effect on K562/AO2, the non-cytotoxic concentration was 5 μg/mL, the momordica at non-cytotoxic concentration could partly reverse the MDR of K562/AO2 cell lines to adriamycin, vincristine, daunomycin (5.4, 6.5 and 4.0 times). The apoptosis of the K562/AO2 cell lines were induced at the 5 μg/mL momordica combined with vincristine, and the apoptosis rate was (19.38 ± 1.06) % . The apoptosis rate of the control group, sin-gle momordica group, single vincristine group were (1.64 ± 0.27)%, (3.79 ± 0.82)%, (9.83 ± 0.98) % , respectively. CONCLUSION: Momordica could partly reverse the MDR of K562/AO2 cell lines in different groups such as adriamycin, vincristine and daunomycin.A certain dose of momordica combined with vincristine could increase the apoptosis rate of tu-mour cells.关键词
苦瓜蛋白/K562/AO2细胞/多药耐药Key words
momordica/ K562/AO2 cell/ multi-drug resistant分类
医药卫生引用本文复制引用
叶爱芳,尹丽慧,熊术道,韩义香,章圣辉,吴建波..苦瓜蛋白逆转K562/AO2细胞多药耐药性的研究[J].中国临床药理学与治疗学,2009,14(7):766-769,4.基金项目
浙江省中医药青年基金项目(2006Y013) （2006Y013）
