中国临床药理学与治疗学2009，Vol.14Issue(1)：10-13,4.
大鼠相关脑区p-CREB和c-Fos在吗啡点燃条件性位置偏爱条件下的变化
Changes of p-CREB and c-Fos expression in brain regions of morphine-reactlvated conditioned place preference in rats
摘要
Abstract
AIM: To investigate the changes of phospho-cAMP response element binding protein (p-CREB) and c-Fos in two brain regions of morphine-reactivated conditioned place preference (CPP) in rats. METHODS: Morphine was administered by subcutaneous injection at gradually increasing dose for 6 days to establish morphine CPP. From the 7th day, the rats were administered saline instead of morphine for 10 days to induce CPP extinction. The rats were given a single priming injection of morphine to reactivate the morphine CPP. The p-CREB and c-Fos were assayed with immunohistochemistry method in the phase of recurrence of CPP rekindled by morphine. RESULTS: Increasing dose of morphine conditioning for 6 days resulted in acquisition of CPP and morphine (4 mg/kg) reactivated CPP following 10 days drug free period. Compared with saline control, morphine -reactivated CPP elevated the expression of p-CREB and c-Fos in hippocampus and amygdala in rats ( P < 0.05 ). CONCLUSION: p-CREB and c-Fos expression in hippocampus and amygdala may be involved in the mechanisms of recurrence of CPP.关键词
吗啡/条件性位置偏爱/海马/杏仁核/cAMP反应元件结合蛋白/c-FosKey words
morphine/conditioned place preference/hippocampus/amygdala/cAMP response element binding protein/c-Fos分类
医药卫生引用本文复制引用
赵永娜,方正梅,邵晓霞,李晓红,李顺英..大鼠相关脑区p-CREB和c-Fos在吗啡点燃条件性位置偏爱条件下的变化[J].中国临床药理学与治疗学,2009,14(1):10-13,4.基金项目
云南省教育厅科学技术基金项目(03Y480C) （03Y480C）
云南省应用基础研究计划面上项目(2006C0037Q) （2006C0037Q）
