中国免疫学杂志2009，Vol.25Issue(11)：969-972,4.
Anti-DR5 mAb诱导佐剂型关节炎大鼠滑膜细胞凋亡及机理初探
Induction of apoptosis by Anti-DR5 mAb in synovial cells of adjuvant arthritis rats and its possible mechanisms
摘要
Abstract
Objective:To study the effects of Anti-DR5 mAb on inducing synovial cells of adjuvant arthritis (AA) rats and its mechanisms.Methods:AA was induced by CFA in rats.The synovial cells of rats were separated and cultured in vitro system.The apoptosis induced by anti-DR5 mAb on synovial cells was measured by MTT analysis,DNA fragmentation and flow cytometry.Caspase-3 and Bcl-2 expression in synovial cells were detected by Western blot.The involvement of the apoptotic pathway was further proved by a caspase inhibition assay.Results:MTT result showed that Anti-DR5 mAb could inhibit synovial cells growth.And flow cytometry suggested that the cell death mode was apoptosis.The protein level of caspase-3 in synovial cells treated with anti-DR5 mAb was raised,while Bcl-2 level declined.When the caspase inhibitor was added to the synovial cells treated with anti-DR5 mAb,it was showed in a dose-dependence inhibition effect,indicating that anti-DR5 mAb inducing apoptosis might be through the caspase pathway.Conclusion:Anti-DR5 mAb could induce synovial cell apoptosis through caspase pathway.And this effect may be related to the protein level of Caspase-3 and Bcl-2.关键词
Anti-DR5 mAb/佐剂型关节炎/滑膜细胞/细胞凋亡Key words
Anti-DR5 mAb/ Adjuvant arthritis/ Synovial cells/ Cell apoptosis分类
医药卫生引用本文复制引用
李文珠,殷平,张佳锴,邱劲华,胡庆中,陶惠然,庄国洪..Anti-DR5 mAb诱导佐剂型关节炎大鼠滑膜细胞凋亡及机理初探[J].中国免疫学杂志,2009,25(11):969-972,4.基金项目
本文为福建省自然科学基金项目(C0710046) （C0710046）
