中国免疫学杂志2009，Vol.25Issue(11)：999-1002,4.
不同途径的供者淋巴细胞输注对移植物抗宿主病的影响
Influence of intra-bone marrow injection of donor lymphocytes upon the development of graft-versus-host disease
毕延智 1曾冬香 1盛桂凤 1赵刚 2陈宝安2
作者信息
- 1. 江苏省常州市肿瘤医院肿瘤内科,常州,213001
- 2. 东南大学附属中大医院血液科,南京,210009
- 折叠
摘要
Abstract
Objective:To investigate the effect of donor lymphocyte infusion (DLI) by intra-bone marrow (IBM) or intravenous (IV) on the incidence of graft-versus-host disease(GVHD) after allogeneic peripheral hematopoietic stem cell transplantation (allo-PBSCT).Methods:Female C57BL/6 mice as recipients received total body irradiation (TBI) on day 0,followed by injection of peripheral hematopoietic stem cells from mobilized male BALB/c with granulocyte-colony stimulating factor (rhG-CSF),and DLI was performed via IV or IBM.The extent of GVHD was compared in recipients received allogeneic IBM-DLI with those received IV-DLI.The percentage of donor-derived cells and CD4~+CD25~+ regulatory T cells (Tregs) was detected by flow cytometry.14 days after DLI,the levels of IL-4 and interferon (IFN)-γ were tested by ELISA.Results:It was found that the frequency and severity of GVHD were reduced in IBM-DLI compared with that of IV-DLI (P＜0.01).7 days after transplantation,the percentage of H-2~d-positive cells was over 95% in all surviving transplanted mice;and 14 days after transplantation,the percentage of Tregs detected as CD4~+CD25~+ was significantly higher in recipients treated with IBM-DLI than those treated with IV-DLI(P＜0.01).Compared with that of the recipients in IBM-DLI group,the level of IL-4 was significantly decreased,while the level of IFN-γ were elevated in group IV-DLI (P＜0.01).Conclusion:IBM-DLI could induce the proliferation of Tregs and the Th polarizing to Th2,resulting in decreasing the incidence and alleviating severity of GVHD after allo-PBSCT.关键词
供者淋巴细胞输注/移植物抗宿主病/细胞因子/调节性T细胞Key words
Donor lymphocyte infusion/ Graft-versus-host disease/ Cytokine/ Regulatory T cells分类
医药卫生引用本文复制引用
毕延智,曾冬香,盛桂凤,赵刚,陈宝安..不同途径的供者淋巴细胞输注对移植物抗宿主病的影响[J].中国免疫学杂志,2009,25(11):999-1002,4.
