中国农业科学2009，Vol.42Issue(11)：4051-4057,7.DOI:10.3864/j.issn.0578-1752.2009.11.036
FSHβ基因外显子2的多态性及其与两个山羊品种繁殖性能的相关性研究
Polymorphism of Exon 2 of FSHβ Gene and Its Relationship with Reproduction Performance in Two Goat Breeds
摘要
Abstract
[Objective] The objectives of the present study were to detect the polymorphism in follicle stimulating hormone beta (FSHβ) and investigate the relationship between FSHβ gene and high prolificacy in goats. [Method] According to the sequence of ovine FSHβ gene, two pairs of primers were designed to detect single nucleotide polymorphism of exon 2 of FSHβ gene in two goat breeds by PCR-SSCE The least square mean and genetic variance of different genotypes at polymorphic loci were analyzed, and the selection reaction was forecasted by genetic correlation between marker locus and genetic variance of litter size traits. [Result] There was one polymorphic locus occurring in exon2 (P_2) with three genotypes (EE, EF and FF). In Xinong Saanen dairy goat, EE genotype had significantly higher (P<0.05) litter size than EF and FF genotype in the first to fourth parity and average parity; EF genotype had significantly higher (P<0.05) litter size than FF genotype in the second and average parity. In Boer goat, EE genotype had significantly higher (P<0.01) litter size than EF and FF genotype in the second to fourth parity and average parity; EF genotype had significantly higher (P<0.05) litter size than FF genotype in average parity. In Xinong Saanen dairy and Boer goat, the heredity of litter size was mainly influenced by genetic additive effect. [Conclusion] In Xinong Saanen dairy and Boer goat, the EE genotype is a favorable marker genotype for litter size.关键词
山羊/卵泡刺激素β亚基/多态性/产羔数Key words
goat/FSHβ/polymorphism/litter size分类
农业科技引用本文复制引用
安小鹏,曹斌云,韩丹,侯金星,李广,王娅娜,李玲,朱广琴,王建刚,宋宇轩..FSHβ基因外显子2的多态性及其与两个山羊品种繁殖性能的相关性研究[J].中国农业科学,2009,42(11):4051-4057,7.基金项目
国家高技术研究发展计划("863")项目(2007AA102167)、国家"十一五"奶业重大科技支撑项目(2006BAD04A11) （"863"）
