中国农业科学2009，Vol.42Issue(11)：4064-4068,5.DOI:10.3864/j.issn.0578-1752.2009.11.038
热应激对猪睾丸细胞活性氧水平影响
Effects of Heat Stress on Reactive Oxygen Species in Swine Testicular Cells
摘要
Abstract
[Objective] To study the effect of heat stress on oxidative injuries in swine testicular cells. [Method] Swine testicular cells were divided into 6 groups, 43℃ 1 h, 43℃ 2 h, 43℃ 1 h+37℃ 6 h, 43℃ 2 h+37℃ 6 b, negative and positive groups. Cellular and mitoehondrial morphology were observed by using light microscope and transmission electron microscope. Laser confocal microscopy and flow eytometry were used to determine the level of reactive oxygen species (ROS) in testicular cells with ROS test Kit. [Result] After 1 h with 43℃ treatment, cells became circular and mitochondria showed partial or extensive denaturation, especially obvious in 43℃ 2 h and 43℃ 2 h+37℃ 6 h groups. There were no significant increase of ROS in testicular cells in 43℃ 1 h group (P>0.05), but the level of ROS obviously increased in 43℃ 2 h group, compared to the former (P<0.05), and the level of ROS in 43℃ 1 h and 43℃ 2 h groups also continued to increase after additional 6 h in 37℃, compared to negative group (P<0.05). [Conclusion] Heat stress could result in the morphological changes of mitoehondria of testicular cells and induce oxidative injuries, thus could affect the reproductive function of testis.关键词
热应激/睾丸细胞/活性氧/线粒体Key words
heat stress/testicular cells/reactive oxygen spoeies/mitochondria分类
农业科技引用本文复制引用
张晓峰,刘迪,王淼,李垚..热应激对猪睾丸细胞活性氧水平影响[J].中国农业科学,2009,42(11):4064-4068,5.基金项目
中国博士后科学基金(20080440834)、哈尔滨市科技局青年科技创新人才基金(2008RFQXS083) （20080440834）
